公开(公告)号：US08207301B2

公开(公告)日：2012-06-26

申请号：US12452216

申请日：2008-06-16

申请人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

发明人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

IPC分类号： C07K14/435

CPC分类号： C07K14/4748 ,  G01N33/57438

摘要： Provided are: a method of assessing hepatocellular carcinoma by using a protein with a different phosphorylated state in hepatocellular carcinoma cells compared with non-hepatocellular carcinoma cells; and a hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma formed of the protein. The hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma includes tumor rejection antigen gp96 formed of the amino acid represented by SEQ ID NO: 1, and is measured for its phosphorylated state to detect the presence or absence of hepatocellular carcinoma.

摘要翻译： 提供：与非肝细胞癌细胞相比，通过使用肝细胞癌细胞中具有不同磷酸化状态的蛋白质来评估肝细胞癌的方法; 和用于检测由蛋白质形成的肝细胞癌的肝细胞癌蛋白标记物。 用于检测肝细胞癌的肝细胞癌蛋白标志物包括由SEQ ID NO：1表示的氨基酸形成的肿瘤排斥抗原gp96，并测量其磷酸化状态以检测肝细胞癌的存在或不存在。

公开(公告)号：US08053066B2

公开(公告)日：2011-11-08

申请号：US11887844

申请日：2007-01-18

申请人： Masahiro Yamakawa ,  Kenji Miyazaki

发明人： Masahiro Yamakawa ,  Kenji Miyazaki

IPC分类号： B32B7/02

CPC分类号： H05K1/092 ,  H01B1/16 ,  Y10T428/24942

摘要： A conductive paste contains metal powder, an inorganic binder, and an organic vehicle as main ingredients. The organic vehicle includes a solvent having a boiling point of 270° C. or higher, and the proportion of the solvent with respect to the entire solvent included in the organic vehicle is 3 to 100% by weight.

摘要翻译： 导电糊包含金属粉末，无机粘合剂和有机载体作为主要成分。 有机载体包括沸点为270℃以上的溶剂，溶剂相对于有机载体中所含的全部溶剂的比例为3〜100重量％。

公开(公告)号：US07778540B2

公开(公告)日：2010-08-17

申请号：US11790782

申请日：2007-04-27

申请人： Kenji Miyazaki ,  Takeo Miki ,  Junji Miura ,  Takahisa Nakano ,  Takashi Yamaguchi ,  Shinya Tokuda

发明人： Kenji Miyazaki ,  Takeo Miki ,  Junji Miura ,  Takahisa Nakano ,  Takashi Yamaguchi ,  Shinya Tokuda

IPC分类号： G03B15/02 ,  H04N5/445 ,  H04N1/21 ,  H04N1/23

CPC分类号： G03H1/22 ,  G03H1/26 ,  G03H1/265 ,  G03H2001/2223 ,  G03H2001/2247 ,  G03H2001/2273 ,  G03H2222/34 ,  G03H2227/06 ,  G07D7/0032 ,  G07D7/121

摘要： On a recording medium serving as a verification object, a picture whose state changes in accordance with optical conditions is formed. A verification apparatus images a plurality of pictures of the recording medium while changing an optical relationship among a light source for illuminating the recording medium, a camera for imaging a picture of the recording medium, and the recording medium with a condition modifying unit. The verification apparatus displays the plurality of pictures of the recording medium imaged by the camera, on the display unit in a state in which the optical relationship has been changed. The verification apparatus determines imaging conditions for imaging a picture for verification on the basis of the optical relationship at the time of photographing selected pictures among the plurality of pictures displayed on the display unit.

摘要翻译： 在用作验证对象的记录介质上，形成其状态根据光学条件而变化的图像。 验证装置在改变用于照亮记录介质的光源之间的光学关系，用于对记录介质的图像进行成像的相机和具有条件修改单元的记录介质之间的光学关系的同时，对记录介质的多个图像进行成像。 验证装置在光学关系已经改变的状态下在显示单元上显示由相机成像的记录介质的多张图像。 验证装置基于在显示单元上显示的多个图像中拍摄所选择的图像时的光学关系，确定用于对图像进行成像的成像条件。

公开(公告)号：US07670841B2

公开(公告)日：2010-03-02

申请号：US10536824

申请日：2003-11-28

申请人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Takuji Nabetani

发明人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Takuji Nabetani

IPC分类号： G01N33/00

CPC分类号： G01N33/6842 ,  G01N33/6821

摘要： The present invention provides, as a method of analyzing the C-terminal amino acid sequence of a peptide with use of reaction technique for successively releasing the C-terminal amino acids, in which undesirable side reactions, such as cleavage of a peptide bond at the middle of the peptide, can be prevented and chemical treatments therein can be carried out under widely applicable conditions in the course of successive release of the C-terminal amino acids from a peptide, such a method comprising steps of dehydrating the gel on which a target peptide that has been separated by gel electrophoresis is held in the bound state; immersing it in a mixture solution of an alkanoic acid anhydride added with a small amount of a perfluoroalkanoic acid in a dipolar aprotic solvent to re-swell the gel carrier, forming a 5-oxazolone structure, at a temperature chosen in the range of from 30° C. to 80° C., followed by the cleavage of the 5-oxazolone ring to release the C-terminal amino acids, and then specifying the C-terminal amino acid sequence of the peptide based on the measured decrease in the molecular weight of a series of reaction products resulting therefrom.

摘要翻译： 本发明提供了使用用于连续释放C-末端氨基酸的反应技术分析肽的C-末端氨基酸序列的方法，其中不期望的副反应，例如在 肽的中间，可以在广泛适用的条件下在肽的C末端氨基酸连续释放的过程中进行其中的化学处理，这种方法包括使凝胶脱水，其中将目标 通过凝胶电泳分离的肽保持在结合状态; 将其加入到加入少量全氟链烷酸的链烷酸与偶极非质子传递溶剂的混合溶液中，使溶胶载体重新溶胀，形成5-恶唑酮结构，温度范围为30℃ ℃至80℃，然后切割5-恶唑酮环以释放C末端氨基酸，然后根据测定的分子量减少指定肽的C-末端氨基酸序列 的一系列由此产生的反应产物。

公开(公告)号：US07243843B2

公开(公告)日：2007-07-17

申请号：US10995540

申请日：2004-11-24

申请人： Takeo Miki ,  Kenji Miyazaki ,  Takashi Yamaguchi ,  Shinya Tokuda

发明人： Takeo Miki ,  Kenji Miyazaki ,  Takashi Yamaguchi ,  Shinya Tokuda

IPC分类号： G06K5/00

CPC分类号： G06T1/0028 ,  G06Q20/341 ,  G06Q20/3552 ,  G06Q20/40145 ,  G06T2201/0051 ,  G07C9/00031 ,  G07C2209/41 ,  G07F7/1008

摘要： In a personal authentication medium issuing apparatus, a main image information is input and whether a personal authentication medium on which synthesized image information having sub-information embedded in the main image information is checked or not is determined based on the state of the input main image information. Further, synthesized image information having sub-information embedded in an invisible state in the main image information which is set in a visible state is formed, a personal authentication medium is formed by printing the synthesized image information on a recording medium and the thus formed personal authentication media are sorted into personal authentication media determined to be completely formed and personal authentication media determined to require a checking process.

摘要翻译： 在个人认证介质发行装置中，输入主图像信息，并且基于输入主图像的状态来确定是否检测到具有嵌入在主图像信息中的子信息的合成图像信息被检查的个人认证介质 信息。 此外，形成具有嵌入在可见状态的主图像信息中的不可见状态的子信息的合成图像信息，通过将合成图像信息印刷在记录介质上形成个人认证介质，并且由此形成的个人 认证介质被分类为确定为完全形成的个人认证介质，并且确定要求检查过程的个人认证介质。

公开(公告)号：US07048879B2

公开(公告)日：2006-05-23

申请号：US10310880

申请日：2002-12-06

申请人： Tomoyuki Kobayashi ,  Kenji Miyazaki ,  Eiji Nagara ,  Michiaki Sasayama ,  Mitsuo Okubo

发明人： Tomoyuki Kobayashi ,  Kenji Miyazaki ,  Eiji Nagara ,  Michiaki Sasayama ,  Mitsuo Okubo

IPC分类号： B29C44/06

CPC分类号： B29C44/3484 ,  B29C43/222 ,  B29C43/28 ,  B29C44/00 ,  B29C44/04 ,  B29C44/22 ,  B29C59/005 ,  B29C59/04 ,  B29C59/046 ,  B32B5/18 ,  B32B37/153 ,  B32B38/06 ,  B32B2305/022 ,  B32B2309/02 ,  B32B2309/04 ,  Y10T428/24496 ,  Y10T428/24661 ,  Y10T428/249953 ,  Y10T428/249958 ,  Y10T428/24996 ,  Y10T428/249981

摘要： A sheet-form structure of expandable thermoplastic resin is provided which is capable of undergoing a pseudo-one-dimensional expansion in its thickness direction and which enables manufacture of thermoplastic resin foams having reduced variations in thickness and weight precisions, increased compressive strength, and excellent properties such as surface smoothness. A method of manufacturing the sheet-form structure, as well as such thermoplastic resin foams, are also provided.A sheet-form structure 1 of expandable thermoplastic resin including granules 2 of expandable thermoplastic resin arranged generally uniformly for integral connection thereof through a thin film 3 of expandable thermoplastic resin. A thermoplastic resin foam including a continuous, thermoplastic resin foam layer, a number of highly-expanded, thermoplastic resin portions provided on at least one surface of the continuous foam layer, and slightly-expanded, thermoplastic resin thin films for covering outer surfaces of respective highly-expanded portions.

公开(公告)号：US20060057731A1

公开(公告)日：2006-03-16

申请号：US10538305

申请日：2003-12-04

申请人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Takuji Nabetani

发明人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Takuji Nabetani

IPC分类号： G01N33/00

CPC分类号： G01N33/6821 ,  G01N33/6842

摘要： The present invention provides, as for a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions, a following method wherein a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products by analysis in negative mode of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

摘要翻译： 本发明提供了通过使用连续释放肽的C末端氨基酸的反应来分析肽的C末端氨基酸序列的方法，该方法可以在连续释放C端 具有长氨基酸长度的肽的氨基酸，这种不利的副反应，如在肽的中间位置处的肽键断裂，并且可以在广泛适用的条件下进行化学处理，以下方法，其中将 具有长氨基酸长度的肽预先进行N-酰化处理; 通过使用其中链烷酸酐与少量全氟链烷酸组合的反应试剂，在温和条件下连续释放C-末端氨基酸; 应用水解处理; 然后通过胰蛋白酶消化进行精氨酸残基位点的选择性碎裂; 此后，通过MALDI-TOF-MS装置的负模式的分析，测量衍生自一系列反应产物的C末端侧片段的分子量的降低; 鉴定出肽样品的C-末端氨基酸序列。

公开(公告)号：US20050236182A1

公开(公告)日：2005-10-27

申请号：US10517058

申请日：2003-06-02

申请人： Noriki Hayashi ,  Yoshio Oka ,  Masahiko Kanda ,  Narito Yagi ,  Kenji Miyazaki ,  Kyouichirou Nakatsugi

发明人： Noriki Hayashi ,  Yoshio Oka ,  Masahiko Kanda ,  Narito Yagi ,  Kenji Miyazaki ,  Kyouichirou Nakatsugi

IPC分类号： H05K1/09 ,  H05K3/10 ,  H05K3/12 ,  H05K3/16 ,  H05K3/24 ,  H05K3/38 ,  H05K1/16

CPC分类号： H05K3/246 ,  H05K1/095 ,  H05K3/108 ,  H05K3/12 ,  H05K3/1216 ,  H05K3/16 ,  H05K3/381 ,  H05K3/388 ,  H05K2201/0347 ,  H05K2201/035 ,  H05K2203/072 ,  H05K2203/095 ,  Y10T29/49126

摘要： A novel board for printed wiring comprising a fine conductor wiring having a clear and favorable boundary line and fabricated by an ordinal printing method such as screen printing, a printed wiring board using the same, and methods for manufacturing them. A board for printed wiring and a method for manufacturing the same are characterized in that the surface of a board is subjected to one of the surface treatments: (a) roughening, (2) plasma treatment, (3) roughening and then plasma treatment, and (4) roughening and then forming of a metal film coating by sputtering. A printed wiring board and a method for manufacturing the same is characterize in that a conductor wiring is fabricated by printing using a conductive paste containing metal particles the average particle diameter of which is 4 μm or less and the maximum particle diameter of which is 15 μm or less. Another printed wiring board and a method for manufacturing the same is characterized in that the surface of a conductor wiring fabricated using a conductive paste containing metal particles M and a binder B at a volume ratio of M/B of 1/1 to 1.9/1 is etched, a plating coating is formed on the surface.

摘要翻译： 一种用于印刷布线的新型印刷电路板，包括具有透明且有利的边界线的精细导体布线，并通过诸如丝网印刷的顺序印刷法，使用其的印刷线路板及其制造方法制造。 印刷电路板及其制造方法的特征在于，对板的表面进行表面处理之一：（a）粗糙化，（2）等离子体处理，（3）粗糙化，然后进行等离子体处理， 和（4）通过溅射粗化然后形成金属膜涂层。 印刷电路板及其制造方法的特征在于，使用含有平均粒径为4μm以下，最大粒径为15μm的金属粒子的导电糊进行印刷，制造导体布线 或更少。 另一种印刷线路板及其制造方法的特征在于，使用含有金属粒子M和粘合剂B的M / B的体积比为1/1〜1.9 / 1的导电性糊剂制造的导体布线的表面 被蚀刻，在表面上形成镀覆层。

公开(公告)号：US20050074832A1

公开(公告)日：2005-04-07

申请号：US10489198

申请日：2003-03-24

申请人： Kenji Miyazaki ,  Akira Tsugita ,  Naoyuki Takahashi ,  Takuji Nabetani ,  Toshimasa Yamazaki ,  Kenichi Kamijo

发明人： Kenji Miyazaki ,  Akira Tsugita ,  Naoyuki Takahashi ,  Takuji Nabetani ,  Toshimasa Yamazaki ,  Kenichi Kamijo

IPC分类号： G01N27/62 ,  G01N33/68 ,  C12Q1/37 ,  G01N33/00

CPC分类号： G01N33/6821 ,  G01N33/6842

摘要： The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by applying reaction technique for successively releasing the C-terminal amino acids therefrom, which method can suppress, when releasing the C-terminal amino acids of the peptide in sequence, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide, and allows to carry out the chemical treatment thereof under widely applicable conditions. In the method according to the present invention, an alkanoic acid anhydride and a perfluoroalkanoic acid both of vapor phase, which are supplied from a mixture containing an alkanoic acid anhydride with a small amount of a perfluoroalkanoic acid added thereto, are allowed to act on a dry sample of the peptide to be examined in a dry atmosphere at a temperature chosen in a range of 15 to 60° C.; whereby the release of the C-terminal amino acid is resulted from successive formation of a 5-oxazolone structure being followed by cleavage of the 5-oxazolone ring; and then the C-terminal amino acids sequence is identified by analysis based on the decrease in molecular weight in a series of the reaction products obtained.

摘要翻译： 本发明提供了通过应用从其中连续释放C末端氨基酸的反应技术来分析肽的C末端氨基酸序列的方法，当释放肽的C末端氨基酸时，该方法可以抑制 序列，这种不利的副反应，如在肽的中间位置处的肽键断裂，并允许在广泛适用的条件下进行化学处理。 在根据本发明的方法中，允许从含有链烷酸酐和少量全氟链烷酸的混合物中提供的气相的链烷酸酐和全氟烷酸作用于 在干燥气氛中在15至60℃范围内选择的温度下待检测的肽的干样品; 由此C末端氨基酸的释放是由连续形成5-恶唑酮结构而引起的，随后是5-恶唑酮环的裂解; 然后通过基于所获得的一系列反应产物中分子量的降低的分析来鉴定C-末端氨基酸序列。

公开(公告)号：US06620495B1

公开(公告)日：2003-09-16

申请号：US09639889

申请日：2000-08-17

申请人： Tomoyuki Kobayashi ,  Kenji Miyazaki ,  Eiji Nagara ,  Michiaki Sasayama ,  Mitsuo Okubo

发明人： Tomoyuki Kobayashi ,  Kenji Miyazaki ,  Eiji Nagara ,  Michiaki Sasayama ,  Mitsuo Okubo

IPC分类号： B32B312

CPC分类号： B29C44/3484 ,  B29C44/00 ,  B29C44/04 ,  Y10T428/24496 ,  Y10T428/24661 ,  Y10T428/249953 ,  Y10T428/24996 ,  Y10T428/249981

摘要： A sheet-form structure of expandable thermoplastic resin is provided which is capable of undergoing a pseudo-one-dimensional expansion in its thickness direction and which enables manufacture of thermoplastic resin foams having reduced variations in thickness and weight precisions, increased compressive strength, and excellent properties such as surface smoothness. A method of manufacturing the sheet-form structure, as well as such thermoplastic resin foams, are also provided. A sheet-form structure 1 of expandable thermoplastic resin including granules 2 of expandable thermoplastic resin arranged generally uniformly for integral connection thereof through a thin film 3 of expandable thermoplastic resin. A thermoplastic resin foam including a continuous, thermoplastic resin foam layer, a number of highly-expanded, thermoplastic resin portions provided on at least one surface of the continuous foam layer, and slightly-expanded, thermoplastic resin thin films for covering outer surfaces of respective highly-expanded portions.

摘要翻译： 提供了一种可膨胀热塑性树脂的片状结构，其能够在其厚度方向上进行假一维膨胀，并且能够制造厚度和重量精度变化减小，抗压强度提高以及优异的热塑性树脂泡沫 性质如表面平滑度。 还提供了一种制造片状结构体的方法以及这种热塑性树脂发泡体。1.一种可膨胀热塑性树脂的片状结构体1，其包括通常均匀地布置的可膨胀热塑性树脂的颗粒2，用于通过薄膜 3的可膨胀热塑性树脂。 一种热塑性树脂发泡体，包括连续的热塑性树脂泡沫层，设置在连续泡沫层的至少一个表面上的多个高度膨胀的热塑性树脂部分，以及用于覆盖相应的外部表面的略微膨胀的热塑性树脂薄膜 高度扩张部分。