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公开(公告)号：US20080182327A1
公开(公告)日：2008-07-31
申请号：US11558856
申请日：2006-11-10
CPC分类号： C12N15/74 , C12N15/635 , C12N15/68
摘要： Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including the E. coli relBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such system are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollutant degrading organisms or as biological pest control organisms e.g. expressing B. thuringiensis crystalline proteins.
摘要翻译： 有条件地控制重组细胞群的生存能力和将这些细胞包含在宿主细胞环境或含有复制子中的方法是基于使用包括大肠杆菌relBE基因座在内的蛋白质杀伤系统和在革兰氏阴性菌中发现的类似系统 和革兰氏阳性菌和古菌。 这种系统通常基于细胞毒素多肽和与细胞毒素相反的抗毒素或解毒剂多肽可被蛋白酶降解。 重组细胞可用作疫苗，污染物降解生物体或作为生物有害生物控制生物体，例如， 表达苏云金芽孢杆菌结晶蛋白。
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公开(公告)号：US20080206840A1
公开(公告)日：2008-08-28
申请号：US11837456
申请日：2007-08-10
IPC分类号： C12N1/20
CPC分类号： C12N15/74 , C12N15/635 , C12N15/68
摘要： Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including E. coli relBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such systems are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollutant degrading organisms or as biological pest control organism e.g. expressing B. thuringiensis crystalline proteins.
摘要翻译： 有条件地控制重组细胞群的生存性和将这些细胞包含在宿主细胞的环境或含有复制子的方法是基于使用包括大肠杆菌relBE基因座在内的蛋白质杀伤系统和在革兰氏阴性和 革兰氏阳性菌和古菌。 这样的系统通常基于细胞毒素多肽和与细胞毒素相反的抗毒素或解毒剂多肽可被蛋白酶降解。 重组细胞可用作疫苗，污染物降解生物体或作为生物有害生物控制生物体。 表达苏云金芽孢杆菌结晶蛋白。
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公开(公告)号：US07595186B2
公开(公告)日：2009-09-29
申请号：US11837456
申请日：2007-08-10
IPC分类号： C12N1/20
CPC分类号： C12N15/74 , C12N15/635 , C12N15/68
摘要： Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including E. coli relBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such systems are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollutant degrading organisms or as biological pest control organism e.g. expressing B. thuringiensis crystalline proteins.
摘要翻译： 有条件地控制重组细胞群的生存性和将这些细胞包含在宿主细胞的环境或含有复制子的方法是基于使用包括大肠杆菌relBE基因座在内的蛋白质杀伤系统和在革兰氏阴性和 革兰氏阳性菌和古菌。 这样的系统通常基于细胞毒素多肽和与细胞毒素相反的抗毒素或解毒剂多肽可被蛋白酶降解。 重组细胞可用作疫苗，污染物降解生物体或作为生物有害生物控制生物体。 表达苏云金芽孢杆菌结晶蛋白。
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公开(公告)号：US07595185B2
公开(公告)日：2009-09-29
申请号：US11558856
申请日：2006-11-10
IPC分类号： C12N1/20
CPC分类号： C12N15/74 , C12N15/635 , C12N15/68
摘要： Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including the E. coli relBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such system are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollutant degrading organisms or as biological pest control organisms e.g. expressing B. thuringiensis crystalline proteins.
摘要翻译： 有条件地控制重组细胞群的生存能力和将这些细胞包含在宿主细胞环境或含有复制子中的方法是基于使用包括大肠杆菌relBE基因座在内的蛋白质杀伤系统和在革兰氏阴性菌中发现的类似系统 和革兰氏阳性菌和古菌。 这种系统通常基于细胞毒素多肽和与细胞毒素相反的抗毒素或解毒剂多肽可被蛋白酶降解。 重组细胞可用作疫苗，污染物降解生物体或作为生物有害生物控制生物体，例如， 表达苏云金芽孢杆菌结晶蛋白。
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公开(公告)号：US07183097B1
公开(公告)日：2007-02-27
申请号：US09700130
申请日：1999-05-07
IPC分类号： C12N1/20
CPC分类号： C12N15/74 , C12N15/635 , C12N15/68
摘要： Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including the E. coli relBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such system are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollulant degrading organisms or as biogical pest control organisms e.g. expressing B. thuringiensis crystalline proteins.
摘要翻译： 有条件地控制重组细胞群的生存能力和将这些细胞包含在宿主细胞环境或含有复制子中的方法是基于使用包括大肠杆菌relBE基因座在内的蛋白质杀伤系统和在革兰氏阴性菌中发现的类似系统 和革兰氏阳性菌和古菌。 这种系统通常基于细胞毒素多肽和与细胞毒素相反的抗毒素或解毒剂多肽可被蛋白酶降解。 重组细胞可用作疫苗，污染物降解生物体或作为生物传播的有害生物控制生物体。 表达苏云金芽孢杆菌结晶蛋白。
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6.发明申请Plasmids from an extremely thermophilic microorganism and derived expression vectors 审中-公开来自嗜热微生物的质粒和衍生的表达载体公开(公告)号：US20050026293A1
公开(公告)日：2005-02-03
申请号：US10778935
申请日：2004-02-13
申请人： Birgitte Ahring , Anders Clausen , Marie Mikkelsen
发明人： Birgitte Ahring , Anders Clausen , Marie Mikkelsen
IPC分类号： C07K14/195 , C12N1/21 , C12N9/12 , C12N15/63 , C12N15/74
CPC分类号： C12N9/1252 , C07K14/195 , C12N15/63 , C12N15/74
摘要： The present invention concerns the isolation of plasmids from extremely thermophilic anaerobic microorganisms and their use in genetic transformation of thermophilic and mesophilic microorganisms. More particular the invention concerns the use of thermostable plasmid vectors as tools for creating shuttle vectors for genetic transformation of extremely thermophilic anaerobic microorganisms.
摘要翻译： 本发明涉及从嗜温厌氧微生物中分离质粒及其在嗜热和嗜温微生物遗传转化中的用途。 更具体地，本发明涉及使用热稳定性质粒载体作为用于产生穿梭载体用于极度嗜热厌氧微生物的遗传转化的工具。
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