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    Diagnostic test for detecting anti-cashew IgE in patients
    2.
    发明授权
    Diagnostic test for detecting anti-cashew IgE in patients 有权
    检测患者抗腰果IgE的诊断试验

    公开(公告)号：US08114605B1

    公开(公告)日：2012-02-14

    申请号：US12025875

    申请日：2008-02-05

    申请人： Kenneth H. Roux ,  Shridhar K. Sathe ,  Suzanne S. Teuber

    发明人： Kenneth H. Roux ,  Shridhar K. Sathe ,  Suzanne S. Teuber

    IPC分类号： G01N33/53

    CPC分类号： C07K14/415 ,  A61K38/10 ,  A61K38/168 ,  A61K45/06 ,  A61K49/0004 ,  C07K7/08 ,  G01N33/6854 ,  G01N2333/415 ,  Y10S435/81

    摘要： Disclosed are major allergenic proteins in cashew nut, which are legumin-like proteins and 2S albumins. Also disclosed is a polypeptide allergen in the 7S superfamily, which includes vicilin-like and sucrose binding proteins. Several linear epitopes of the cashew nut are identified and characterized. The invention further discloses the sequence of cDNA encoding the allergenic polypeptide, the allergen being designated Ana o 1, and also describes the characterization of the expressed recombinant polypeptide and associated methods employing the polypeptide.

    摘要翻译： 披露了腰果中的主要过敏原蛋白，其是豆科蛋白样蛋白和2S白蛋白。 还公开了7S超家族中的多肽过敏原，其包括vicilin样和蔗糖结合蛋白。 确定和表征腰果的几个线性表位。 本发明进一步公开了编码变应原性多肽的cDNA序列，变应原命名为Ana o1，并描述了表达的重组多肽的表征和使用该多肽的相关方法。

    Purified linear polypeptide epitope from walnuts and associated methods
    8.
    发明授权
    Purified linear polypeptide epitope from walnuts and associated methods 失效
    从核桃和相关方法纯化的线性多肽表位

    公开(公告)号：US07250489B2

    公开(公告)日：2007-07-31

    申请号：US10251918

    申请日：2002-09-20

    申请人： Kenneth H. Roux ,  Shridhar K. Sathe ,  Jason M. Robotham ,  Suzanne S. Teuber

    发明人： Kenneth H. Roux ,  Shridhar K. Sathe ,  Jason M. Robotham ,  Suzanne S. Teuber

    IPC分类号： A61K38/04 ,  C07K5/00 ,  C07K7/00

    CPC分类号： C07K14/415 ,  A61K39/00 ,  G01N33/564

    摘要： A purified Ig-E binding immunogenic polypeptide the amino acid sequence of which consists of SEQ ID NO:1 is disclosed. The purified amino acid sequence is a major allergen of the walnut Juglans regia. Also described are a diagnostic test kit and use of the purified polypeptide in tests for identifying patients having an allergy to walnuts. Mutations of the disclosed polypeptide, as well as nucleic acid sequences encoding therefor are claimed as part of the invention.

    摘要翻译： 公开了由SEQ ID NO：1组成的氨基酸序列的纯化的Ig-E结合免疫原性多肽。 纯化的氨基酸序列是核桃胡桃核桃的主要变应原。 还描述了诊断测试试剂盒和在用于鉴定对核桃过敏的患者的测试中使用纯化的多肽。 公开的多肽的突变以及编码的核酸序列被要求保护作为本发明的一部分。

    Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts (Anacardium occidentale)
    9.
    发明授权
    Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts (Anacardium occidentale) 有权
    在腰果（西葫芦）中编码的核酸和过敏原多肽

    公开(公告)号：US07537914B1

    公开(公告)日：2009-05-26

    申请号：US10529602

    申请日：2003-11-04

    申请人： Kenneth Roux ,  Shridhar K. Sathe ,  Jason M. Robotham ,  Suzanne S. Teuber

    发明人： Kenneth Roux ,  Shridhar K. Sathe ,  Jason M. Robotham ,  Suzanne S. Teuber

    IPC分类号： C12P21/02 ,  C12N1/21 ,  C12N15/03 ,  C07K14/415 ,  C07H21/04

    CPC分类号： G01N33/6854 ,  C07K14/415 ,  Y10S435/81

    摘要： The invention describes an isolated nucleic acid sequence comprising the nucleotide sequence of SEQ ID NO:1 or a degenerate variant of SEQ ID NO:1. The nucleic acid sequence encodes an Ig-E binding immunogenic polypeptide the amino acid sequence of which comprises at least one sequence selected from SEQ ID NOS:4-25. The invention additionally provides an in vitro diagnostic test for detecting anti-cashew IgE in a patient. The test comprises reacting the patient's serum with a purified polypeptide the amino acid sequence of which comprises at least one sequence selected from SEQ ID NOS:3-25; separating the polypeptide from unreacted patient serum; reacting the polypeptide with a labeled human IgE-reactive agent after separating from unreacted patient serum; separating the polypeptide from unreacted labeled human IgE-reactive agent; and detecting labeled human IgE-reactive agent bound to the polypeptide after separating from unreacted agent to thereby indicate presence in the patient's serum of anti-cashew IgE.

    摘要翻译： 本发明描述了分离的核酸序列，其包含SEQ ID NO：1的核苷酸序列或SEQ ID NO：1的简并变体。 核酸序列编码Ig-E结合免疫原性多肽，其氨基酸序列包含至少一个选自SEQ ID NO：4-25的序列。 本发明另外提供用于检测患者抗腰果IgE的体外诊断试验。 测试包括使患者的血清与纯化的多肽反应，其纯化多肽的氨基酸序列包含至少一个选自SEQ ID NO：3-25的序列; 将多肽与未反应的患者血清分离; 在从未反应的患者血清中分离后，使多肽与标记的人IgE反应剂反应; 将多肽与未反应的标记的人IgE反应剂分离; 以及在从未反应剂分离后检测与多肽结合的标记的人IgE反应剂，从而指示患者血清中抗腰果IgE的存在。

    Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts
    10.
    发明申请
    Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts 失效
    在腰果中编码的核酸和变应原多肽

    公开(公告)号：US20090092959A1

    公开(公告)日：2009-04-09

    申请号：US11906801

    申请日：2007-10-04

    申请人： Kenneth Roux ,  Shridhar K. Sathe ,  Jason M. Robotham ,  Suzanne S. Teuber

    发明人： Kenneth Roux ,  Shridhar K. Sathe ,  Jason M. Robotham ,  Suzanne S. Teuber

    IPC分类号： C12Q1/00 ,  C07K7/00

    CPC分类号： G01N33/6854 ,  C07K14/415 ,  Y10S435/81

    摘要： The invention describes an isolated nucleic acid sequence comprising the nucleotide sequence of SEQ ID NO:1 or a degenerate variant of SEQ ID NO: 1. The nucleic acid sequence encodes an Ig-E binding immunogenic polypeptide the amino acid sequence of which comprises at least one sequence selected from SEQ ID NOS:4-25. The invention additionally provides an in vitro diagnostic test for detecting anti-cashew IgE in a patient. The test comprises reacting the patient's serum with a purified polypeptide the amino acid sequence of which comprises at least one sequence selected from SEQ ID NOS:3-25; separating the polypeptide from unreacted patient serum; reacting the polypeptide with a labeled human IgE-reactive agent after separating from unreacted patient serum; separating the polypeptide from unreacted labeled human IgE-reactive agent; and detecting labeled human IgE-reactive agent bound to the polypeptide after separating from unreacted agent to thereby indicate presence in the patient's serum of anti-cashew IgE.

    摘要翻译： 本发明描述了分离的核酸序列，其包含SEQ ID NO：1的核苷酸序列或SEQ ID NO：1的简并变体。核酸序列编码Ig-E结合免疫原性多肽，其氨基酸序列至少包含 一个选自SEQ ID NO：4-25的序列。 本发明另外提供用于检测患者抗腰果IgE的体外诊断试验。 测试包括使患者的血清与纯化的多肽反应，其纯化多肽的氨基酸序列包含至少一个选自SEQ ID NO：3-25的序列; 将多肽与未反应的患者血清分离; 在从未反应的患者血清中分离后，使多肽与标记的人IgE反应剂反应; 将多肽与未反应的标记的人IgE反应剂分离; 以及在从未反应剂分离后检测与多肽结合的标记的人IgE反应剂，从而指示患者血清中抗腰果IgE的存在。