公开(公告)号：US20170114405A1

公开(公告)日：2017-04-27

申请号：US15400920

申请日：2017-01-06

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia van EIJK ,  Adrianus Johannes van TUNEN

IPC: C12Q1/68 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/13 ,  G06F19/22 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

公开(公告)号：US20170159120A1

公开(公告)日：2017-06-08

申请号：US15434801

申请日：2017-02-16

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia van EIJK ,  Adrianus Johannes van TUNEN

IPC: C12Q1/68 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/13 ,  G06F19/22 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

公开(公告)号：US20170166962A1

公开(公告)日：2017-06-15

申请号：US15434788

申请日：2017-02-16

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia van EIJK ,  Adrianus Johannes van TUNEN

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/13 ,  G16B30/00 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

公开(公告)号：US20160258006A1

公开(公告)日：2016-09-08

申请号：US15165921

申请日：2016-05-26

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia van EIJK ,  Adrianus Johannes van TUNEN

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/13 ,  G06F19/22 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.