公开(公告)号：US20150232924A1

公开(公告)日：2015-08-20

申请号：US14626822

申请日：2015-02-19

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Henricus Johannes Adam Van der Poel

IPC: C12Q1/68 ,  G06F19/22

CPC classification number: G06F19/22 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156 ,  C40B30/04 ,  C40B30/10 ,  C12Q2539/107 ,  C12Q2531/113 ,  C12Q2525/191

Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.

公开(公告)号：US09453256B2

公开(公告)日：2016-09-27

申请号：US15009304

申请日：2016-01-28

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Henricus Johannes Adam Van der Poel

IPC: C40B30/04 ,  C12Q1/68 ,  C12N15/10 ,  G06F19/22 ,  C40B30/10

CPC classification number: G06F19/22 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156 ,  C40B30/04 ,  C40B30/10 ,  C12Q2539/107 ,  C12Q2531/113 ,  C12Q2525/191

Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.

公开(公告)号：US09493820B2

公开(公告)日：2016-11-15

申请号：US14626822

申请日：2015-02-19

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Henricus Johannes Adam Van der Poel

IPC: C40B30/04 ,  C12Q1/68 ,  C12N15/10 ,  G06F19/22 ,  C40B30/10

CPC classification number: G06F19/22 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156 ,  C40B30/04 ,  C40B30/10 ,  C12Q2539/107 ,  C12Q2531/113 ,  C12Q2525/191

Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.