公开(公告)号：US20070231794A1

公开(公告)日：2007-10-04

申请号：US11232479

申请日：2005-09-21

申请人： Kilian Dill ,  John Cooper ,  Andrei Ghindilis ,  Kristian Roth

发明人： Kilian Dill ,  John Cooper ,  Andrei Ghindilis ,  Kristian Roth

IPC分类号： C12Q1/68 ,  G01N33/567 ,  G06F19/00

CPC分类号： C12Q1/001 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N33/5438 ,  C12Q2565/607 ,  C12Q2563/125 ,  C12Q2537/149

摘要： The present invention provides a process to detect binding events on an electrode microarray. A microarray is provided having addressable electrodes and two or more different types of capture complexes at sites corresponding to the electrodes. The capture complexes capture analytes. Enzymes are attached to form a reporter complex. Substrate solutions are sequentially contacted to make enzyme products that are detectable at the electrodes by a difference in the electrical response at electrodes having the enzyme product and those not having the enzyme product. The enzyme product may be a solid deposition product. The electrical properties of electrodes on the microarray are read for the presence of the enzyme product by sequentially switching each electrode held at a constant voltage to ground and then back to the constant voltage.

摘要翻译： 本发明提供一种检测电极微阵列上的结合事件的方法。 提供了一种微阵列，其具有可寻址电极和两个或多个不同类型的俘获复合物在对应于电极的位置。 捕获复合物捕获分析物。 酶结合形成报告复合物。 依次接触底物溶液，以通过在具有酶产物的电极和不具有酶产物的电极上的电响应差异在电极处检测到酶产物。 酶产物可以是固体沉积产物。 通过将保持在恒定电压的每个电极顺序地切换到地，然后回到恒定电压，读取微阵列上的电极的电学性质以获得酶产物的存在。