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1.发明申请Low Polysaccharide Microorganisms for Production of Biofuels and Other Renewable Materials 审中-公开用于生产生物燃料和其他可再生材料的低多糖微生物公开(公告)号：US20140137463A1
公开(公告)日：2014-05-22
申请号：US13859229
申请日：2013-04-09
申请人： Kirk Apt , Jacob Borden , Paul W. Behrens , David Dain , Jon Hansen , Joseph W. Pfeifer
发明人： Kirk Apt , Jacob Borden , Paul W. Behrens , David Dain , Jon Hansen , Joseph W. Pfeifer
CPC分类号： C12P7/6409 , C10L1/1802 , C11B1/025 , C11C3/10 , C12N1/16 , C12P7/649 , C12R1/645 , F02B43/00 , Y02E50/13 , Y02E50/343 , Y02T10/32
摘要： High cell density fermentations of wild-type organisms can result in increased viscosity due to the production of exocellular polysaccharides. Mutant microorganisms with a dry morphology, resulting from reduced exocellular polysaccharide formation, were isolated and characterized. The exocellular polysaccharide composition for these modified microorganisms is shown to be different than the polysaccharide composition of the wild type microorganism. In addition to reduced exocellular polysaccharide formation, dry morphology mutants of multiple strains show reduced viscosity, improved oxygen mass transfer, and improved fatty acid fermentation yield on carbon.
摘要翻译： 由于外细胞多糖的生成，野生型生物体的高细胞密度发酵可导致粘度增加。 分离和表征了由减少的外细胞多糖形成导致的具有干燥形态的突变微生物。 这些改性微生物的外细胞多糖组合物显示与野生型微生物的多糖组成不同。 除了减少的外细胞多糖形成，多种菌株的干态形态突变体显示出降低的粘度，改善的氧传质，以及改进的碳的脂肪酸发酵产率。
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2.发明授权公开(公告)号：US07745183B2
公开(公告)日：2010-06-29
申请号：US11738414
申请日：2007-04-20
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。
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3.发明授权Production of DHA in microalgae in medium having modified amounts of potassium 有权在具有改性量的钾的培养基中，在微藻中生产DHA公开(公告)号：US07824892B2
公开(公告)日：2010-11-02
申请号：US11738425
申请日：2007-04-20
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。
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公开(公告)号：US08008050B2
公开(公告)日：2011-08-30
申请号：US11738422
申请日：2007-04-20
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。
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公开(公告)号：US07829129B2
公开(公告)日：2010-11-09
申请号：US11738416
申请日：2007-04-20
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。
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公开(公告)号：US07252979B2
公开(公告)日：2007-08-07
申请号：US11313524
申请日：2005-12-20
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。
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7.发明申请Production of High Levels of DHA in Microalgae Using Modified Amounts of Chloride and Potassium 审中-公开使用改性量的氯化物和钾生产微藻中高含量的DHA公开(公告)号：US20120071677A1
公开(公告)日：2012-03-22
申请号：US13306370
申请日：2011-11-29
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。
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8.发明授权Production of DHA in microalgae in medium having modified amounts of chloride ion and potassium ion 失效在具有改变量的氯离子和钾离子的培养基中，在微藻中生产DHA公开(公告)号：US07871809B2
公开(公告)日：2011-01-18
申请号：US11738430
申请日：2007-04-20
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。
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9.发明授权Production of high levels of DHA in microalgae using modified amounts of chloride and potassium 有权使用改良量的氯化物和钾在微藻中生产高水平的DHA公开(公告)号：US07163811B2
公开(公告)日：2007-01-16
申请号：US10957075
申请日：2004-10-01
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。
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公开(公告)号：US20080032355A1
公开(公告)日：2008-02-07
申请号：US11738418
申请日：2007-04-20
申请人： Paul Behrens , John Thompson , Kirk Apt , Joseph Pfeifer , James Wynn , James Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul Behrens , John Thompson , Kirk Apt , Joseph Pfeifer , James Wynn , James Lippmeier , Jaouad Fichtali , Jon Hansen
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
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