公开(公告)号：US5994629A

公开(公告)日：1999-11-30

申请号：US527474

申请日：1995-09-13

申请人： Kirsten Bojsen ,  Iain Donaldson ,  Anna Haldrup ,  Morten Joersboe ,  Jette D. Kreiberg ,  John Nielsen ,  Finn T. Okkels ,  Steen G. Petersen ,  Robert J. Whenham

发明人： Kirsten Bojsen ,  Iain Donaldson ,  Anna Haldrup ,  Morten Joersboe ,  Jette D. Kreiberg ,  John Nielsen ,  Finn T. Okkels ,  Steen G. Petersen ,  Robert J. Whenham

IPC分类号： A01H5/12 ,  C07H15/203 ,  C07H15/26 ,  C07H17/02 ,  C07H19/16 ,  C07H19/20 ,  C12N5/00 ,  C12N9/24 ,  C12N9/90 ,  C12N9/92 ,  C12N15/65 ,  C12N15/82 ,  C12N15/00

CPC分类号： C07H15/203 ,  A01H5/12 ,  C07H15/26 ,  C07H17/02 ,  C07H19/16 ,  C07H19/20 ,  C12N15/65 ,  C12N15/8209 ,  C12N15/8245 ,  C12N5/0025 ,  C12N9/2402 ,  C12N9/90 ,  C12N9/92 ,  C12Y302/01031

摘要： A method of selecting genetically transformed cells from a population of cells comprising introducing a desired nucleotide sequence and a co-introduced nucleotide sequence into the genome of a cell whereby the desired nucleotide sequence or the co-introduced nucletoide sequence induces a positive effect by giving the transformed cells a competitive advantage when the population of cells are supplied with an inactive compound thereby allowing the transformed cells to be identified and selected from the non-transformed cells by means defined as positive selection; as well as cells transformed according to the method and plants derived therefrom. The invention further relates to novel glucuronide compounds including cytokinin glucuronide compounds for use in the method.

摘要翻译： 从细胞群中选择遗传转化的细胞的方法，包括将期望的核苷酸序列和共同引入的核苷酸序列引入细胞的基因组，由此所需的核苷酸序列或共同引入的核苷序列通过给予 当细胞群体被供应无活性化合物从而允许通过定义为阳性选择的方式从非转化细胞中鉴定和选择转化的细胞时，转化细胞具有竞争优势; 以及根据方法转化的细胞和从其衍生的植物。 本发明还涉及包含用于该方法的细胞分裂素葡糖苷酸化合物的新型葡糖苷酸化合物。