公开(公告)号：US5238810A

公开(公告)日：1993-08-24

申请号：US915022

申请日：1992-07-15

申请人： Koichi Fujiwara ,  Juichi Noda ,  Hiroko Misutani ,  Hiromichi Mizutani, deceased

发明人： Koichi Fujiwara ,  Juichi Noda ,  Hiroko Misutani ,  Hiromichi Mizutani, deceased

IPC分类号： G01N33/543 ,  G01N35/10

CPC分类号： G01N33/54373 ,  G01N33/54326 ,  G01N2035/1025 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/817

摘要： A laser magnetic immunoassay (LMIA) method which enables detection of less than picograms of target analyte in milliliter of analyte solution. The method involves the steps of: affixing an antibody or antigen on the surface of non-magnetic carrier particles; immunoreacting the affixed antibody or antigen to capture a target analyte contained in a specimen; preparing magnetic labeled microparticles treated so as to bind with the target analyte; reacting the labeled complex to sandwich the target analyte between the magnetic particles and the non-magnetic carrier particles; separating the free species from the bound species by centrifugation; dispersing the precipitated solid to make an analyte solution; applying a spot magnetic field gradient on the analyte solution and irradiating a selected spot with a laser beam; and analyzing the resulting interference patterns to quantitatively determine the quantity of target analyte.

摘要翻译： 激光磁免疫测定（LMIA）方法，可以检测到毫升分析物溶液中少于皮克的目标分析物。 该方法包括以下步骤：将抗体或抗原固定在非磁性载体颗粒的表面上; 免疫反应固定的抗体或抗原以捕获包含在样品中的目标分析物; 制备被处理以与目标分析物结合的磁性标记微粒; 使标记的复合物与磁性颗粒和非磁性载体颗粒之间夹住目标分析物; 通过离心分离游离物种与结合物种; 分散沉淀的固体以制备分析物溶液; 在分析物溶液上施加点磁场梯度并用激光束照射所选择的点; 并分析所得到的干扰图案以量化确定目标分析物的量。