公开(公告)号：US20050221399A1

公开(公告)日：2005-10-06

申请号：US11092944

申请日：2005-03-30

申请人： Koichi Nakano ,  Masaki Ishisaka ,  Kazuki Kishi ,  Miharu Watanabe ,  Yasuyuki Imura

发明人： Koichi Nakano ,  Masaki Ishisaka ,  Kazuki Kishi ,  Miharu Watanabe ,  Yasuyuki Imura

IPC分类号： G01N33/574 ,  G01N33/58 ,  G01N33/567

CPC分类号： G01N33/57411 ,  G01N33/582

摘要： The invention provides a reagent for diagnosing cervical cancer, which can not only detect the presence or absence of cervical cancer but can also distinguish squamous cell carcinoma and adenocarcinoma from each other, a method for screening cervical cancer by using the reagent, particularly a screening method capable high speed processing by utilizing flow cytometry. The reagent comprises a first labeled antibody reacting with gland cells, a second labeled antibody reacting with adenocarcinoma cells and a third labeled antibody reacting with atypical cervical squamous cells, the antibodies being labeled with mutually distinguishable labels respectively. Preferably, at least one selected from the group consisting of MUC1 antibody, cytokeratin 7 antibody, and cytokeratin 18 antibody is used as the first labeled antibody, at least one selected from the group consisting of cytokeratin 8 antibody and HIK1083 antibody is used as the second labeled antibody, and at least one member selected from the group consisting of NMP179 antibody, p16INK4A antibody, Ki-67 antibody, p53 antibody, p21 antibody, EMA antibody, CEA antibody and MIB-1 antibody is used as the third labeled antibody.

摘要翻译： 本发明提供了一种用于诊断宫颈癌的试剂，其不仅可以检测宫颈癌的存在或不存在，还可以区分鳞状细胞癌和腺癌，通过使用试剂筛选子宫颈癌的方法，特别是筛选方法 利用流式细胞仪进行高速处理。 试剂包含与腺体细胞反应的第一标记抗体，与腺癌细胞反应的第二标记抗体和与非典型宫颈鳞状细胞反应的第三标记抗体，所述抗体分别用相互区分的标记标记。 优选使用选自MUC1抗体，细胞角蛋白7抗体和细胞角蛋白18抗体的至少一种作为第一标记抗体，使用选自细胞角蛋白8抗体和HIK1083抗体的至少一种作为第二标记抗体 标记抗体，选自NMP179抗体，p16，INK4A抗体，Ki-67抗体，p53抗体，p21抗体，EMA抗体，CEA抗体和MIB-1抗体中的至少一种是 用作第三标记抗体。