公开(公告)号：US20050221399A1

公开(公告)日：2005-10-06

申请号：US11092944

申请日：2005-03-30

申请人： Koichi Nakano ,  Masaki Ishisaka ,  Kazuki Kishi ,  Miharu Watanabe ,  Yasuyuki Imura

发明人： Koichi Nakano ,  Masaki Ishisaka ,  Kazuki Kishi ,  Miharu Watanabe ,  Yasuyuki Imura

IPC分类号： G01N33/574 ,  G01N33/58 ,  G01N33/567

CPC分类号： G01N33/57411 ,  G01N33/582

摘要： The invention provides a reagent for diagnosing cervical cancer, which can not only detect the presence or absence of cervical cancer but can also distinguish squamous cell carcinoma and adenocarcinoma from each other, a method for screening cervical cancer by using the reagent, particularly a screening method capable high speed processing by utilizing flow cytometry. The reagent comprises a first labeled antibody reacting with gland cells, a second labeled antibody reacting with adenocarcinoma cells and a third labeled antibody reacting with atypical cervical squamous cells, the antibodies being labeled with mutually distinguishable labels respectively. Preferably, at least one selected from the group consisting of MUC1 antibody, cytokeratin 7 antibody, and cytokeratin 18 antibody is used as the first labeled antibody, at least one selected from the group consisting of cytokeratin 8 antibody and HIK1083 antibody is used as the second labeled antibody, and at least one member selected from the group consisting of NMP179 antibody, p16INK4A antibody, Ki-67 antibody, p53 antibody, p21 antibody, EMA antibody, CEA antibody and MIB-1 antibody is used as the third labeled antibody.

摘要翻译： 本发明提供了一种用于诊断宫颈癌的试剂，其不仅可以检测宫颈癌的存在或不存在，还可以区分鳞状细胞癌和腺癌，通过使用试剂筛选子宫颈癌的方法，特别是筛选方法 利用流式细胞仪进行高速处理。 试剂包含与腺体细胞反应的第一标记抗体，与腺癌细胞反应的第二标记抗体和与非典型宫颈鳞状细胞反应的第三标记抗体，所述抗体分别用相互区分的标记标记。 优选使用选自MUC1抗体，细胞角蛋白7抗体和细胞角蛋白18抗体的至少一种作为第一标记抗体，使用选自细胞角蛋白8抗体和HIK1083抗体的至少一种作为第二标记抗体 标记抗体，选自NMP179抗体，p16，INK4A抗体，Ki-67抗体，p53抗体，p21抗体，EMA抗体，CEA抗体和MIB-1抗体中的至少一种是 用作第三标记抗体。

公开(公告)号：US09625388B2

公开(公告)日：2017-04-18

申请号：US12762703

申请日：2010-04-19

申请人： Masaki Ishisaka ,  Kazuki Kishi ,  Masakazu Fukuda

发明人： Masaki Ishisaka ,  Kazuki Kishi ,  Masakazu Fukuda

IPC分类号： G01N21/64 ,  G01N15/14 ,  G01N15/00

CPC分类号： G01N21/6486 ,  G01N15/147 ,  G01N2015/0092 ,  G01N2015/1497

摘要： A cell analysis apparatus that can accurately distinguish between an aggregating cell and a non-aggregating cell is provided. The cell analysis apparatus (10) includes: an optical detection section (3) for flowing a measurement sample obtained from a biological sample and a pigment into a flow cell (51), irradiating the measurement sample flowing in the flow cell (51) with laser beam and detecting fluorescence from the measurement sample; a signal processing circuit (4) for acquiring, based on a fluorescence signal outputted from the detection section (3), a value reflecting the height of a waveform of the signal and a value reflecting the length of a ridge line of the waveform of the signal; and a system control section (13) for distinguishing between an aggregating cell formed by aggregation of a plurality of cells and a non-aggregating cell, based on the value reflecting the height of the waveform of the fluorescence signal and the value reflecting the length of the ridge line of the waveform of the fluorescence signal obtained by the signal processing circuit (4).

公开(公告)号：US07871783B2

公开(公告)日：2011-01-18

申请号：US11966318

申请日：2007-12-28

申请人： Yuichi Yasuda ,  Masakatsu Morita ,  Junyi Ding ,  Rieko Goto ,  Kazuki Kishi

发明人： Yuichi Yasuda ,  Masakatsu Morita ,  Junyi Ding ,  Rieko Goto ,  Kazuki Kishi

IPC分类号： G01N33/53

CPC分类号： G01N33/54393

摘要： A method of activating an antigen is herein described. The method comprises providing an antigen activation solution and contacting the cell with the solution to activate the antigen. A method of detecting a cell fixed by a nonbridging fixation solution is also described. The method comprises providing an antigen activation solution, contacting the cell with the solution, immunostaining the cell, and detecting the stained cell. The solution used for these methods comprises an agent for breaking a hydrogen bond, and is also described herein.

摘要翻译： 本文描述了激活抗原的方法。 该方法包括提供抗原活化溶液并使细胞与溶液接触以活化抗原。 还描述了检测由非桥接固定液固定的细胞的方法。 该方法包括提供抗原活化溶液，使细胞与溶液接触，免疫染色细胞，检测染色的细胞。 用于这些方法的溶液包括用于破坏氢键的试剂，并且在本文中也描述。

公开(公告)号：US20110263444A1

公开(公告)日：2011-10-27

申请号：US13091716

申请日：2011-04-21

申请人： Shinzaburou NOGUCHI ,  Yasuto NAOI ,  Kazuki KISHI ,  Kengo GOTOH

发明人： Shinzaburou NOGUCHI ,  Yasuto NAOI ,  Kazuki KISHI ,  Kengo GOTOH

IPC分类号： C40B30/02 ,  G06F19/00

CPC分类号： G06F19/20 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158 ,  G06F19/24

摘要： Disclosed is a method for examining prognosis of breast cancer including the steps of: (A) extracting RNA from a specimen collected from a subject, (B) preparing a determination sample using the extracted RNA, (C) determining the expression level of each gene in the specific gene groups using the obtained determination sample, (D) analyzing the expression level of the determined each gene, and (E) examining prognosis of breast cancer, based on the obtained analysis result are performed.

摘要翻译： 本发明公开了一种检查乳腺癌预后的方法，包括以下步骤：（A）从从受试者收集的标本中提取RNA，（B）使用提取的RNA制备测定样品，（C）测定各基因的表达水平 在使用得到的测定样品的特定基因组中，（D）分析所测定的各基因的表达水平，（E）基于所得分析结果检测乳腺癌的预后。

公开(公告)号：US20100196917A1

公开(公告)日：2010-08-05

申请号：US12762703

申请日：2010-04-19

申请人： Masaki Ishisaka ,  Kazuki Kishi ,  Masakazu Fukuda

发明人： Masaki Ishisaka ,  Kazuki Kishi ,  Masakazu Fukuda

IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12Q1/02

CPC分类号： G01N21/6486 ,  G01N15/147 ,  G01N2015/0092 ,  G01N2015/1497

摘要： A cell analysis apparatus that can accurately distinguish between an aggregating cell and a non-aggregating cell is provided. The cell analysis apparatus (10) includes: an optical detection section (3) for flowing a measurement sample obtained from a biological sample and a pigment into a flow cell (51), irradiating the measurement sample flowing in the flow cell (51) with laser beam and detecting fluorescence from the measurement sample; a signal processing circuit (4) for acquiring, based on a fluorescence signal outputted from the detection section (3), a value reflecting the height of a waveform of the signal and a value reflecting the length of a ridge line of the waveform of the signal; and a system control section (13) for distinguishing between an aggregating cell formed by aggregation of a plurality of cells and a non-aggregating cell, based on the value reflecting the height of the waveform of the fluorescence signal and the value reflecting the length of the ridge line of the waveform of the fluorescence signal obtained by the signal processing circuit (4).

摘要翻译： 提供了可以精确地区分聚集单元和非聚集单元的单元分析设备。 细胞分析装置（10）包括：光检测部（3），用于将从生物样品和颜料获得的测定样品流入流动池（51），向流动池（51）中流动的测定样品照射 激光束和检测来自测量样品的荧光; 信号处理电路（4），用于基于从检测部（3）输出的荧光信号，获取反映信号的波形的高度的值和反映该信号的波形的脊线的长度的值 信号; 以及系统控制部（13），用于基于反映荧光信号的波形的高度的值和反映荧光信号的长度的值来区分由多个单元的聚集形成的聚集单元和非聚集单元 由信号处理电路（4）获得的荧光信号波形的脊线。

公开(公告)号：US20080160542A1

公开(公告)日：2008-07-03

申请号：US11966318

申请日：2007-12-28

申请人： Yuichi YASUDA ,  Masakatsu Morita ,  Junyi Ding ,  Rieko Goto ,  Kazuki Kishi

发明人： Yuichi YASUDA ,  Masakatsu Morita ,  Junyi Ding ,  Rieko Goto ,  Kazuki Kishi

IPC分类号： G01N33/53 ,  C12N5/02 ,  C07C275/00

CPC分类号： G01N33/54393

摘要： A method of activating an antigen is herein described. The method comprises providing an antigen activation solution and contacting the cell with the solution to activate the antigen. A method of detecting a cell fixed by a nonbridging fixation solution is also described. The method comprises providing an antigen activation solution, contacting the cell with the solution, immunostaining the cell, and detecting the stained cell. The solution used for these methods comprises an agent for breaking a hydrogen bond, and is also described herein.

摘要翻译： 本文描述了激活抗原的方法。 该方法包括提供抗原活化溶液并使细胞与溶液接触以活化抗原。 还描述了检测由非桥接固定液固定的细胞的方法。 该方法包括提供抗原活化溶液，使细胞与溶液接触，免疫染色细胞，检测染色的细胞。 用于这些方法的溶液包括用于破坏氢键的试剂，并且在本文中也描述。

公开(公告)号：US20080108103A1

公开(公告)日：2008-05-08

申请号：US11906083

申请日：2007-09-28

申请人： Masaki Ishisaka ,  Yasuyuki Imura ,  Kazuki Kishi

发明人： Masaki Ishisaka ,  Yasuyuki Imura ,  Kazuki Kishi

IPC分类号： C12Q1/20 ,  C12M1/34

CPC分类号： G01N15/1459 ,  G01N15/1463 ,  G01N21/51 ,  G01N21/6428 ,  G01N21/645 ,  G01N2015/1477 ,  G01N2021/4707

摘要： Highly accurate and easy discrimination of cancer/atypical cells is achieved. A cancer/atypical cell discrimination method comprises: measuring a plurality of cells by a flow cytometer; acquiring a scattered light signal for each of the cells; calculating at least one characteristic parameter by analyzing the waveform of the scattered light signal; and discriminating a cancer/atypical cell from the plurality of cells based on the characteristic parameter.

摘要翻译： 实现对癌症/非典型细胞的高度准确和容易的鉴别。 癌症/非典型细胞鉴别方法包括：通过流式细胞仪测量多个细胞; 获取每个单元的散射光信号; 通过分析散射光信号的波形来计算至少一个特征参数; 以及基于所述特征参数从所述多个单元格区分癌症/非典型细胞。

公开(公告)号：US08855941B2

公开(公告)日：2014-10-07

申请号：US13091716

申请日：2011-04-21

申请人： Shinzaburou Noguchi ,  Yasuto Naoi ,  Kazuki Kishi ,  Kengo Gotoh

发明人： Shinzaburou Noguchi ,  Yasuto Naoi ,  Kazuki Kishi ,  Kengo Gotoh

IPC分类号： G01N33/48 ,  C12Q1/68 ,  G06F19/20 ,  G06F19/24

CPC分类号： G06F19/20 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158 ,  G06F19/24

摘要： Disclosed is a method for examining prognosis of breast cancer including the steps of: (A) extracting RNA from a specimen collected from a subject, (B) preparing a determination sample using the extracted RNA, (C) determining the expression level of each gene in the specific gene groups using the obtained determination sample, (D) analyzing the expression level of the determined each gene, and (E) examining prognosis of breast cancer, based on the obtained analysis result are performed.

摘要翻译： 本发明公开了一种检查乳腺癌预后的方法，包括以下步骤：（A）从从受试者收集的标本中提取RNA，（B）使用提取的RNA制备测定样品，（C）测定各基因的表达水平 在使用得到的测定样品的特定基因组中，（D）分析所测定的各基因的表达水平，（E）基于所得分析结果检测乳腺癌的预后。