公开(公告)号：US20090091746A1

公开(公告)日：2009-04-09

申请号：US12286702

申请日：2008-10-01

申请人： Masakazu Fukuda ,  Masaki Ishisaka

发明人： Masakazu Fukuda ,  Masaki Ishisaka

IPC分类号： G01N21/17 ,  G01N21/01

CPC分类号： G01N15/147 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1477 ,  G01N2021/6439

摘要： The present invention is to present a cell analyzer capable of measuring cells which are approximately 20 to 100 μm in size with high precision via flow cytometry. The cell analyzer 10 comprises: a flow cell 51 in which a measurement sample including a measurement target cell flows; a light source part 53 for irradiating a light on the measurement sample flowing in the flow cell 51; an optical system 52 for forming a beam spot on the measurement sample flowing in the flow cell 51, the beam spot having a diameter of 3˜8 μm in a flow direction of the measurement sample and a diameter of 300˜600 μm in a direction perpendicular to the flow direction of the measurement sample; and a light receiving part 55, 58, 59 for receiving a light from the measurement sample.

摘要翻译： 本发明是提供能够通过流式细胞术以高精度大小测量大约20〜100μm的细胞的细胞分析装置。 细胞分析装置10包括：流动池51，其中包括测量目标细胞的测量样本流动; 用于对在流动池51中流动的测量样品照射光的光源部53; 用于在流动池51中流动的测量样品上形成束斑的光学系统52，在测量样品的流动方向上具有直径为3〜8μm的束斑，在方向上具有300〜600μm的直径 垂直于测量样品的流动方向; 以及用于接收来自测量样品的光的光接收部分55,58,59。

公开(公告)号：US06219476B1

公开(公告)日：2001-04-17

申请号：US09369314

申请日：1999-08-06

申请人： Hideo Kususawa ,  Yasuyuki Imura ,  Masaki Ishisaka

发明人： Hideo Kususawa ,  Yasuyuki Imura ,  Masaki Ishisaka

IPC分类号： G02B632

CPC分类号： G02B27/143 ,  G01N15/14 ,  G02B6/4204 ,  G02B6/4206

摘要： A multiple light source unit includes a plurality of light sources for emitting light beams. There is a condensing lens and the light beams are parallel to an optical axis of the condensing lens. Included is a mirror for directing the light beams from the plurality of light sources to the condensing lens. A light guiding element for receiving the condensed light beams through a light receiving section and for emitting the light beams through a light emitting section is also provided. The light beams from the plurality of light sources are incident, through respective positions on the condensing lens, into the light receiving section of the light guiding element.

摘要翻译： 多个光源单元包括用于发射光束的多个光源。 存在聚光透镜，并且光束平行于聚光透镜的光轴。 包括用于将来自多个光源的光束引导到聚光透镜的反射镜。 还提供了一种用于通过光接收部分接收聚光的光束并用于通过发光部分发射光束的导光元件。 来自多个光源的光束通过聚光透镜上的相应位置入射到光导元件的光接收部分。

公开(公告)号：US20110104744A1

公开(公告)日：2011-05-05

申请号：US12914243

申请日：2010-10-28

申请人： Masatsugu Ozasa ,  Masaki Ishisaka ,  Tokihiro Kosaka ,  Takamichi Naito ,  Takeo Saitou ,  Shigehiro Numada

发明人： Masatsugu Ozasa ,  Masaki Ishisaka ,  Tokihiro Kosaka ,  Takamichi Naito ,  Takeo Saitou ,  Shigehiro Numada

IPC分类号： C12Q1/06 ,  C12M1/34

CPC分类号： G01N15/1475 ,  G01N15/147 ,  G01N2015/1486

摘要： A cell analyzing apparatus, comprising: a parameter obtaining section for obtaining a characteristic parameter from a cell in a measurement sample; an imaging section for capturing an image of the cell in the measurement sample; an analyzing section for counting a cell in which the characteristic parameter meets a predetermined requirement among the cells in the measurement sample as a counting target and generating output data based on a counting result; a display section for displaying an image of the cell meeting the predetermined requirement and the output data; and an input section for receiving an instruction to specify the image displayed on the display section, wherein the analyzing section excludes a cell relevant to the specified image from the counting target and regenerates the output data is disclosed. A cell analyzing method is also disclosed.

摘要翻译： 一种电池分析装置，包括：参数获取部，用于从测量样本中的单元获得特征参数; 用于捕获测量样本中的单元的图像的成像部分; 一个分析部分，用于对特征参数满足预定要求的单元格的单元进行计数，作为计数目标并基于计数结果产生输出数据; 显示单元，用于显示满足预定要求的单元的图像和输出数据; 以及输入部分，用于接收指定显示在显示部分上的图像的指令，其中分析部分从计数目标中排除与指定图像相关的单元，并且重新生成输出数据。 还公开了细胞分析方法。

公开(公告)号：US06289126B1

公开(公告)日：2001-09-11

申请号：US09131375

申请日：1998-08-07

申请人： Masaki Ishisaka

发明人： Masaki Ishisaka

IPC分类号： G06K956

CPC分类号： G06K9/0014 ,  G06K9/48 ,  G06T5/20 ,  G06T7/0012 ,  G06T7/12 ,  G06T2207/10016 ,  G06T2207/30024

摘要： The boundary of an object is determined based on its optically captured image by: (a) converting a density data of each of a plurality of pixels forming the captured image into a binary signal; (b) converting the binary signal into a neighbor pixel state value so that only the pixels located on the boundary r in the interior of the object have a value corresponding to the binary signals of the surrounding pixels; (c) normalizing the neighbor pixel state value using an absolute chain direction value of a previous chain direction; (d) obtaining a relative chain direction value for a pixel located on the boundary of the object in the image relative to the direction from which the chain has proceeded, with reference to the neighbor pixel state value of the pixel; (e) calculating an absolute chain direction value of a next chain direction on the basis of the relative chain direction value; and (f) repeating the steps (c) to (e) for each pixel located on the boundary to trace the boundary of the object for determining the boundary.

摘要翻译： 基于其光学拍摄图像，通过以下方式确定对象的边界：（a）将形成所述拍摄图像的多个像素中的每一个的密度数据转换为二进制信号; （b）将二进制信号转换为相邻像素状态值，使得只有位于物体内部的边界r上的像素具有与周围像素的二进制信号对应的值; （c）使用先前的链方向的绝对链方向值来归一化邻近像素状态值; （d）参考像素的相邻像素状态值，相对于链已经进行的方向，获得位于图像中物体边界上的像素的相对链方向值; （e）基于相对链条方向值计算下一个链条方向的绝对链条方向值; 以及（f）针对位于边界上的每个像素重复步骤（c）至（e），以跟踪对象的边界以确定边界。

公开(公告)号：US08189177B2

公开(公告)日：2012-05-29

申请号：US12892257

申请日：2010-09-28

申请人： Masakazu Fukuda ,  Kanako Nagaoka ,  Masaki Ishisaka

发明人： Masakazu Fukuda ,  Kanako Nagaoka ,  Masaki Ishisaka

IPC分类号： G01N1/00

CPC分类号： G01N1/34 ,  G01N15/0618 ,  G01N15/147 ,  G01N15/1475 ,  G01N35/1095 ,  G01N2015/1006 ,  G01N2015/1493 ,  G01N2035/00475

摘要： A sample preparation apparatus comprising: a detector for detecting a predetermined cell included in a biological sample; a sample preparation section for preparing a measurement sample from the biological sample and a predetermined reagent; and a controller configured to generate, based on a detection result by the detector, concentration information reflecting a concentration of the predetermined cell in the biological sample and to control, based on the concentration information, the amount of the biological sample supplied to the sample preparation section.

摘要翻译： 一种样品制备装置，包括：用于检测生物样品中包含的预定细胞的检测器; 用于从生物样品和预定试剂制备测量样品的样品制备部分; 以及控制器，被配置为基于检测器的检测结果生成反映生物样品中预定细胞浓度的浓度信息，并且基于浓度信息来控制提供给样品制备物的生物样品的量 部分。

公开(公告)号：US20100196917A1

公开(公告)日：2010-08-05

申请号：US12762703

申请日：2010-04-19

申请人： Masaki Ishisaka ,  Kazuki Kishi ,  Masakazu Fukuda

发明人： Masaki Ishisaka ,  Kazuki Kishi ,  Masakazu Fukuda

IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12Q1/02

CPC分类号： G01N21/6486 ,  G01N15/147 ,  G01N2015/0092 ,  G01N2015/1497

摘要： A cell analysis apparatus that can accurately distinguish between an aggregating cell and a non-aggregating cell is provided. The cell analysis apparatus (10) includes: an optical detection section (3) for flowing a measurement sample obtained from a biological sample and a pigment into a flow cell (51), irradiating the measurement sample flowing in the flow cell (51) with laser beam and detecting fluorescence from the measurement sample; a signal processing circuit (4) for acquiring, based on a fluorescence signal outputted from the detection section (3), a value reflecting the height of a waveform of the signal and a value reflecting the length of a ridge line of the waveform of the signal; and a system control section (13) for distinguishing between an aggregating cell formed by aggregation of a plurality of cells and a non-aggregating cell, based on the value reflecting the height of the waveform of the fluorescence signal and the value reflecting the length of the ridge line of the waveform of the fluorescence signal obtained by the signal processing circuit (4).

摘要翻译： 提供了可以精确地区分聚集单元和非聚集单元的单元分析设备。 细胞分析装置（10）包括：光检测部（3），用于将从生物样品和颜料获得的测定样品流入流动池（51），向流动池（51）中流动的测定样品照射 激光束和检测来自测量样品的荧光; 信号处理电路（4），用于基于从检测部（3）输出的荧光信号，获取反映信号的波形的高度的值和反映该信号的波形的脊线的长度的值 信号; 以及系统控制部（13），用于基于反映荧光信号的波形的高度的值和反映荧光信号的长度的值来区分由多个单元的聚集形成的聚集单元和非聚集单元 由信号处理电路（4）获得的荧光信号波形的脊线。

公开(公告)号：US20080108103A1

公开(公告)日：2008-05-08

申请号：US11906083

申请日：2007-09-28

申请人： Masaki Ishisaka ,  Yasuyuki Imura ,  Kazuki Kishi

发明人： Masaki Ishisaka ,  Yasuyuki Imura ,  Kazuki Kishi

IPC分类号： C12Q1/20 ,  C12M1/34

CPC分类号： G01N15/1459 ,  G01N15/1463 ,  G01N21/51 ,  G01N21/6428 ,  G01N21/645 ,  G01N2015/1477 ,  G01N2021/4707

摘要： Highly accurate and easy discrimination of cancer/atypical cells is achieved. A cancer/atypical cell discrimination method comprises: measuring a plurality of cells by a flow cytometer; acquiring a scattered light signal for each of the cells; calculating at least one characteristic parameter by analyzing the waveform of the scattered light signal; and discriminating a cancer/atypical cell from the plurality of cells based on the characteristic parameter.

摘要翻译： 实现对癌症/非典型细胞的高度准确和容易的鉴别。 癌症/非典型细胞鉴别方法包括：通过流式细胞仪测量多个细胞; 获取每个单元的散射光信号; 通过分析散射光信号的波形来计算至少一个特征参数; 以及基于所述特征参数从所述多个单元格区分癌症/非典型细胞。

公开(公告)号：US20110014646A1

公开(公告)日：2011-01-20

申请号：US12892257

申请日：2010-09-28

申请人： Masakazu Fukuda ,  Kanako Nagaoka ,  Masaki Ishisaka

发明人： Masakazu Fukuda ,  Kanako Nagaoka ,  Masaki Ishisaka

IPC分类号： C12Q1/06 ,  C12M1/34

CPC分类号： G01N1/34 ,  G01N15/0618 ,  G01N15/147 ,  G01N15/1475 ,  G01N35/1095 ,  G01N2015/1006 ,  G01N2015/1493 ,  G01N2035/00475

摘要： A sample preparation apparatus comprising: a detector for detecting a predetermined cell included in a biological sample; a sample preparation section for preparing a measurement sample from the biological sample and a predetermined reagent; and a controller configured to generate, based on a detection result by the detector, concentration information reflecting a concentration of the predetermined cell in the biological sample and to control, based on the concentration information, the amount of the biological sample supplied to the sample preparation section.

摘要翻译： 一种样品制备装置，包括：用于检测生物样品中包含的预定细胞的检测器; 用于从生物样品和预定试剂制备测量样品的样品制备部分; 以及控制器，被配置为基于检测器的检测结果生成反映生物样品中预定细胞浓度的浓度信息，并且基于浓度信息来控制提供给样品制备物的生物样品的量 部分。

公开(公告)号：US20050221399A1

公开(公告)日：2005-10-06

申请号：US11092944

申请日：2005-03-30

申请人： Koichi Nakano ,  Masaki Ishisaka ,  Kazuki Kishi ,  Miharu Watanabe ,  Yasuyuki Imura

发明人： Koichi Nakano ,  Masaki Ishisaka ,  Kazuki Kishi ,  Miharu Watanabe ,  Yasuyuki Imura

IPC分类号： G01N33/574 ,  G01N33/58 ,  G01N33/567

CPC分类号： G01N33/57411 ,  G01N33/582

摘要： The invention provides a reagent for diagnosing cervical cancer, which can not only detect the presence or absence of cervical cancer but can also distinguish squamous cell carcinoma and adenocarcinoma from each other, a method for screening cervical cancer by using the reagent, particularly a screening method capable high speed processing by utilizing flow cytometry. The reagent comprises a first labeled antibody reacting with gland cells, a second labeled antibody reacting with adenocarcinoma cells and a third labeled antibody reacting with atypical cervical squamous cells, the antibodies being labeled with mutually distinguishable labels respectively. Preferably, at least one selected from the group consisting of MUC1 antibody, cytokeratin 7 antibody, and cytokeratin 18 antibody is used as the first labeled antibody, at least one selected from the group consisting of cytokeratin 8 antibody and HIK1083 antibody is used as the second labeled antibody, and at least one member selected from the group consisting of NMP179 antibody, p16INK4A antibody, Ki-67 antibody, p53 antibody, p21 antibody, EMA antibody, CEA antibody and MIB-1 antibody is used as the third labeled antibody.

摘要翻译： 本发明提供了一种用于诊断宫颈癌的试剂，其不仅可以检测宫颈癌的存在或不存在，还可以区分鳞状细胞癌和腺癌，通过使用试剂筛选子宫颈癌的方法，特别是筛选方法 利用流式细胞仪进行高速处理。 试剂包含与腺体细胞反应的第一标记抗体，与腺癌细胞反应的第二标记抗体和与非典型宫颈鳞状细胞反应的第三标记抗体，所述抗体分别用相互区分的标记标记。 优选使用选自MUC1抗体，细胞角蛋白7抗体和细胞角蛋白18抗体的至少一种作为第一标记抗体，使用选自细胞角蛋白8抗体和HIK1083抗体的至少一种作为第二标记抗体 标记抗体，选自NMP179抗体，p16，INK4A抗体，Ki-67抗体，p53抗体，p21抗体，EMA抗体，CEA抗体和MIB-1抗体中的至少一种是 用作第三标记抗体。

公开(公告)号：US09625388B2

公开(公告)日：2017-04-18

申请号：US12762703

申请日：2010-04-19

申请人： Masaki Ishisaka ,  Kazuki Kishi ,  Masakazu Fukuda

发明人： Masaki Ishisaka ,  Kazuki Kishi ,  Masakazu Fukuda

IPC分类号： G01N21/64 ,  G01N15/14 ,  G01N15/00

CPC分类号： G01N21/6486 ,  G01N15/147 ,  G01N2015/0092 ,  G01N2015/1497

摘要： A cell analysis apparatus that can accurately distinguish between an aggregating cell and a non-aggregating cell is provided. The cell analysis apparatus (10) includes: an optical detection section (3) for flowing a measurement sample obtained from a biological sample and a pigment into a flow cell (51), irradiating the measurement sample flowing in the flow cell (51) with laser beam and detecting fluorescence from the measurement sample; a signal processing circuit (4) for acquiring, based on a fluorescence signal outputted from the detection section (3), a value reflecting the height of a waveform of the signal and a value reflecting the length of a ridge line of the waveform of the signal; and a system control section (13) for distinguishing between an aggregating cell formed by aggregation of a plurality of cells and a non-aggregating cell, based on the value reflecting the height of the waveform of the fluorescence signal and the value reflecting the length of the ridge line of the waveform of the fluorescence signal obtained by the signal processing circuit (4).