公开(公告)号：US20170045452A1

公开(公告)日：2017-02-16

申请号：US15305416

申请日：2015-04-15

Applicant: KONICA MINOLTA, INC.

Inventor： Masaru TAKAHASHI ,  Fuminori OKADA

IPC: G01N21/64 ,  C09K11/06 ,  C09K11/02 ,  G01N33/545 ,  C08K5/3437

CPC classification number: G01N21/6428 ,  C08K5/3437 ,  C09K11/025 ,  C09K11/06 ,  C09K2211/1416 ,  C09K2211/1433 ,  G01N33/545 ,  G01N33/582 ,  G01N2021/6439

Abstract: An object of the present invention is to provide a medium which is capable of inhibiting precipitation and/or aggregation of fluorescent dye-containing resin particles and enables to use the fluorescent dye-containing resin particles for staining after a long-term storage without having to perform complicated operations. The present invention provides a medium for storing fluorescent dye-containing resin particles, wherein, in a particle-containing liquid obtained by adding fluorescent dye-containing resin particles to the medium, the rate of change in the backscatter intensity (transmitted light) at the center of the height of the particle-containing liquid left to stand for 24 hours after the addition is not less than −1% based on the particle-containing liquid immediately after the addition.

Abstract translation: 本发明的目的是提供一种能够抑制含荧光染料的树脂粒子的析出和/或聚集的介质，能够在长期保存后能够使用含有荧光染料的树脂粒子进行染色，而不必 执行复杂的操作。 本发明提供了一种用于储存含荧光染料的树脂颗粒的介质，其中在通过向介质中加入含荧光染料的树脂颗粒而获得的含颗粒的液体中，在所述介质中的反向散射强度（透射光）的变化率 相对于添加后立即含有颗粒的液体，添加后的放置24小时的含有颗粒的液体的高度的中心不小于-1％。

公开(公告)号：US20160011179A1

公开(公告)日：2016-01-14

申请号：US14771988

申请日：2014-03-04

Applicant: KONICA MINOLTA, INC.

Inventor： Masaru TAKAHASHI ,  Kensaku TAKANASHI

IPC: G01N33/533 ,  G01N33/52 ,  G01N1/30

CPC classification number: G01N33/533 ,  C08L101/00 ,  C09B67/001 ,  C09B67/0017 ,  G01N1/30 ,  G01N21/6428 ,  G01N33/52 ,  G01N2001/302 ,  G01N2021/6441 ,  G01N2458/00 ,  G01N2800/7028

Abstract: An object of the present invention is to provide a fluorophore having excellent initial emission intensity and light resistance, which is not easily discolored and does not affect the evaluation of the number of bright spots even when it is slightly discolored. The resin particle for fluorescence labeling is characterized by comprising a fluorescent dye immobilized in a resin particle, the fluorescent dye satisfying both of the following conditions (1) and (2): (1) the fluorescent dye alone in an aqueous solution has a concentration-dependent peak emission intensity in a range of 30 to 80 μM; and (2) the emission intensity at a concentration of 100 μM is not less than 80% of the peak emission intensity. It is preferred that the Stoke's shift of the fluorescent dye in an aqueous solution is not less than 25 nm. The fluorescent dye may be subjected to a solubilization treatment (such as an acid treatment). It is preferred that the fluorescent dye be encapsulated in a resin particle comprising a thermosetting resin.

Abstract translation: 本发明的目的是提供一种具有优异的初始发光强度和耐光性的荧光团，即使稍微变色，也不容易变色，也不会影响亮点数的评价。 用于荧光标记的树脂颗粒的特征在于包含固定在树脂颗粒中的荧光染料，满足以下条件（1）和（2）两者的荧光染料：（1）在水溶液中单独的荧光染料具有浓度 相关的峰值发射强度在30至80微米的范围内; （2）浓度为100μM时的发光强度不低于峰值发光强度的80％。 优选的是Stoke在水溶液中荧光染料的移动不小于25nm。 荧光染料可以进行溶解处理（例如酸处理）。 荧光染料优选包封在包含热固性树脂的树脂颗粒中。

公开(公告)号：US20200003778A1

公开(公告)日：2020-01-02

申请号：US16481013

申请日：2018-02-02

Applicant: Konica Minolta, Inc.

Inventor： Masaru TAKAHASHI ,  Takeshi SHIRAISHI ,  Noboru KOYAMA

IPC: G01N33/574 ,  G01N33/58

Abstract: The present invention relates to a method for detecting an antibody-drug-conjugate and relates to a method for determining the efficacy of an antibody-drug-conjugate with high accuracy by a quantitative technique for identifying an expression level of a target molecule in a target cell of the antibody-drug-conjugate and interactions therebetween. According to the method, visualizing a drug and an antibody, or components of an antibody-drug-conjugate, by immunostaining with a phosphor integrated dot enables detection of the antibody-drug-conjugate and the components.

公开(公告)号：US20200003690A1

公开(公告)日：2020-01-02

申请号：US16561732

申请日：2019-09-05

Applicant: KONICA MINOLTA, INC.

Inventor： Masaru TAKAHASHI ,  Fuminori OKADA

IPC: G01N21/64 ,  G01N33/545 ,  C08K5/3437 ,  C09K11/02 ,  C09K11/06

Abstract: A method of storing fluorescent dye-containing resin particles comprising adding the fluorescent dye-containing resin particles in a liquid comprising a buffer, a protein, and a surfactant, thereby obtaining a particle-containing liquid. The rate of change in the backscatter intensity (transmitted light) at the center of the height of the particle-containing liquid left to stand for 24 hours after the adding is not less than −1% based on the particle-containing liquid immediately after the adding.

公开(公告)号：US20230069563A1

公开(公告)日：2023-03-02

申请号：US17979920

申请日：2022-11-03

Applicant: KONICA MINOLTA, INC.

Inventor： Masaru TAKAHASHI ,  Fuminori OKADA

IPC: G01N21/64 ,  G01N33/545 ,  G01N21/59 ,  C08K5/3437 ,  C09K11/02 ,  C09K11/06

Abstract: A particle-containing liquid having a medium and fluorescent dye-containing resin particles, wherein, in the particle-containing liquid obtained by adding fluorescent dye-containing resin particles to the medium, the rate of change in the backscatter intensity (transmitted light) at the center of the height of the particle-containing liquid left to stand for 24 hours after said addition is not less than −1% based on the particle-containing liquid immediately after said addition, and wherein the fluorescent dye-containing resin particles have a particle size ranging from 40 nm to 200 nm.

公开(公告)号：US20220003641A1

公开(公告)日：2022-01-06

申请号：US17280174

申请日：2019-10-09

Applicant: Konica Minolta, Inc.

Inventor： Rie SAKURAGI ,  Masaru TAKAHASHI ,  Shin NAKAYAMA ,  Shumpei HITOSUGI

IPC: G01N1/30 ,  C09K11/06 ,  C09K11/02 ,  C09B67/08 ,  C09B67/20

Abstract: A light-emitting colorant-containing particle contains an organic resin and a light-emitting colorant. A content of the light-emitting colorant is in a range of 10 to 80 mol % based on a total amount of a monomer forming the organic resin and the light-emitting colorant. The particle has an average particle diameter in a range of 1 to 100 nm.

公开(公告)号：US20210381982A1

公开(公告)日：2021-12-09

申请号：US17407302

申请日：2021-08-20

Applicant: KONICA MINOLTA, INC.

Inventor： Masaru TAKAHASHI ,  Fuminori OKADA

IPC: G01N21/64 ,  G01N33/545 ,  G01N21/59 ,  C08K5/3437 ,  C09K11/02 ,  C09K11/06

Abstract: An object of the present invention is to provide a medium which is capable of inhibiting precipitation and/or aggregation of fluorescent dye-containing resin particles and enables to use the fluorescent dye-containing resin particles for staining after a long-term storage without having to perform complicated operations. The present invention provides a medium for storing fluorescent dye-containing resin particles, wherein, in a particle-containing liquid obtained by adding fluorescent dye-containing resin particles to the medium, the rate of change in the backscatter intensity (transmitted light) at the center of the height of the particle-containing liquid left to stand for 24 hours after the addition is not less than −1% based on the particle-containing liquid immediately after the addition.

公开(公告)号：US20210318323A1

公开(公告)日：2021-10-14

申请号：US17273262

申请日：2019-09-05

Applicant: KONICA MINOLTA INC.

Inventor： Kenji NISHIKAWA ,  Masaru TAKAHASHI

IPC: G01N33/574 ,  G01N1/30

Abstract: Information useful for diagnosis or treatment is acquired by quantitatively evaluating the expression levels of each of a plurality of mutually different domains in the same protein in a human tissue slice, by providing: a staining process of using a plurality of staining reagents having mutually different colorings to stain the plurality of different domains in the same protein; a quantifying step of quantifying the expression levels of each of the plurality of domains; and a calculating step of calculating the ratios between the expression levels of the plurality of domains.

公开(公告)号：US20190079081A1

公开(公告)日：2019-03-14

申请号：US16084374

申请日：2017-03-01

Applicant: Konica Minolta, Inc.

Inventor： Masaru TAKAHASHI ,  Yasuyuki MOTOKUI

IPC: G01N33/533 ,  G01N33/536 ,  G01N33/58 ,  G01N1/30

Abstract: A fluorescent immunostaining method including a first reaction step of specifically binding and fixing primary antibodies 1a, 1b to an antigen P as a staining subject and a second reaction step of specifically binding a secondary antibody 2a that has been modified by fluorescent nanoparticle 5 or is to be subsequently modified thereby, to the bound and fixed primary antibodies 1a, 1b in which plural secondary antibodies 2a are fixed to the antigen P by way of the primary antibodies 1a, 1b, making it possible to detect lowly-expressed antigens.

公开(公告)号：US20180136212A1

公开(公告)日：2018-05-17

申请号：US15806970

申请日：2017-11-08

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Masaru TAKAHASHI ,  Kensaku TAKANASHI ,  Hisatake OKADA ,  Yuichi OZAKI ,  Yuka YOSHIHARA ,  Yasushi NAKANO ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE ,  Norikazu MASUDA ,  Masakazu TOI ,  Hiroshi TADA ,  Minoru MIYASHITA

IPC: G01N33/574 ,  G06K9/00

CPC classification number: G01N33/57415 ,  G01N1/30 ,  G01N33/5023 ,  G01N33/582 ,  G01N2333/71 ,  G06K9/00134 ,  G06K9/0014 ,  G06K9/00147

Abstract: The present invention relates to a test support method which is a means for predicting whether or not pathological complete response (pCR) will be attained when a preoperative chemotherapy with an anticancer drug is performed, the method using a sample (breast cancer tissue section) collected from a breast cancer patient to be subjected to the preoperative chemotherapy. The test support method includes: [1] the step of acquiring a fluorescence image of a breast cancer tissue section, which fluorescence image shows bright spots of fluorescent nanoparticles labeling one or more kinds of breast cancer-related proteins; [2] the step of acquiring at least one index relating to the expression level(s) of the breast cancer-related protein(s) on the basis of the bright spots of the fluorescence image; and [3] the step of acquiring information for predicting pCR by performing an analysis using the above-described at least one index.