公开(公告)号：US20180136212A1

公开(公告)日：2018-05-17

申请号：US15806970

申请日：2017-11-08

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Masaru TAKAHASHI ,  Kensaku TAKANASHI ,  Hisatake OKADA ,  Yuichi OZAKI ,  Yuka YOSHIHARA ,  Yasushi NAKANO ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE ,  Norikazu MASUDA ,  Masakazu TOI ,  Hiroshi TADA ,  Minoru MIYASHITA

IPC: G01N33/574 ,  G06K9/00

CPC classification number: G01N33/57415 ,  G01N1/30 ,  G01N33/5023 ,  G01N33/582 ,  G01N2333/71 ,  G06K9/00134 ,  G06K9/0014 ,  G06K9/00147

Abstract: The present invention relates to a test support method which is a means for predicting whether or not pathological complete response (pCR) will be attained when a preoperative chemotherapy with an anticancer drug is performed, the method using a sample (breast cancer tissue section) collected from a breast cancer patient to be subjected to the preoperative chemotherapy. The test support method includes: [1] the step of acquiring a fluorescence image of a breast cancer tissue section, which fluorescence image shows bright spots of fluorescent nanoparticles labeling one or more kinds of breast cancer-related proteins; [2] the step of acquiring at least one index relating to the expression level(s) of the breast cancer-related protein(s) on the basis of the bright spots of the fluorescence image; and [3] the step of acquiring information for predicting pCR by performing an analysis using the above-described at least one index.

公开(公告)号：US20210311024A1

公开(公告)日：2021-10-07

申请号：US15999859

申请日：2017-02-15

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Takeshi SHIRAISHI ,  Takeshi ISODA ,  Noboru KOYAMA ,  Hisatake OKADA

IPC: G01N33/50 ,  G01N33/574

Abstract: An object of the present invention is to provide a non-clinical test method which allows for the quality control of experimental animals having a transplanted tumor site, such as tumor-bearing mice, or experimental animals having a lesion site other than a transplanted a tumor site, and which is characterized by including the step of identifying, using a specimen collected from such an experimental animal, the profile of a lesion site, for example, a transplanted tumor site, of the experimental animal, by a quantitative technique.

公开(公告)号：US20210404919A1

公开(公告)日：2021-12-30

申请号：US17469225

申请日：2021-09-08

Applicant: Konica Minolta, Inc.

Inventor： Takuji AIMIYA ,  Hideki GOUDA ,  Hisatake OKADA ,  Yasushi NAKANO ,  Kohsuke GONDA ,  Motohiro TAKEDA ,  Noriaki OHUCHI

IPC: G01N1/30 ,  G01N33/58 ,  G01N21/64 ,  B82Y30/00 ,  B82Y15/00 ,  G01N33/74

Abstract: A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.

公开(公告)号：US20180024059A1

公开(公告)日：2018-01-25

申请号：US15549006

申请日：2015-02-10

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Kensaku TAKANASHI ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G01N21/64 ,  G01N33/53 ,  G06T7/90 ,  G01N33/50 ,  G01N33/58 ,  G06T7/00 ,  G01N21/93

CPC classification number: G01N21/6428 ,  G01N21/64 ,  G01N21/6486 ,  G01N21/93 ,  G01N33/48 ,  G01N33/483 ,  G01N33/5023 ,  G01N33/53 ,  G01N33/582 ,  G06T7/0016 ,  G06T7/90 ,  G06T2207/30024

Abstract: A biological substance quantitation method includes the following. A fluorescent image is input, which represents an expression of a specific biological substance in a sample stained with a fluorescent substance by a fluorescent bright spot. A quantitative evaluation value of the fluorescent bright spot is calculated. A standard fluorescent image of a standard sample stained under a same condition as the sample and representing an expression of the biological substance in a standard sample, is input under a same condition as the fluorescent image. A quantitative evaluation value in the standard fluorescent image is calculated under a same condition as the fluorescent image. Based on a correlation between an expression amount of the biological substance in a standard sample measured in advance and the evaluation value in the standard fluorescent image, the evaluation value in the fluorescent image is converted to an expression amount of the biological substance in the sample.

公开(公告)号：US20170016911A1

公开(公告)日：2017-01-19

申请号：US15124557

申请日：2015-03-23

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Takeshi ISODA ,  Yasuhiro WATANABE ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G01N33/68 ,  G01N15/14 ,  G01N21/64 ,  G01N1/30 ,  G01N33/58

CPC classification number: G01N33/6872 ,  G01N1/30 ,  G01N15/1429 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/582 ,  G01N2001/302 ,  G01N2021/6439 ,  G01N2496/80

Abstract: The present invention provides a method capable of more accurately quantifying a biological material expressed on the cell membrane in pathological samples. The present invention is directed to a method for quantifying a biological material (target biological material) expressed on the cell membrane, the method including the steps of: (1a) immunostaining the target biological material with a fluorescent material; (1b) immunostaining another biological material (reference biological material) on the cell membrane with another fluorescent material; (2) using immunostaining images for the target and reference biological materials to identify the fluorescence signal corresponding to the target biological material and to measure the fluorescence signals corresponding to the target and reference biological materials; and (3) correcting the measured value of the fluorescence signal corresponding to the target biological material by a given method to quantify the expression level.

Abstract translation: 本发明提供能够更准确地定量病理样品中在细胞膜上表达的生物材料的方法。 本发明涉及一种量化在细胞膜上表达的生物材料（靶生物材料）的方法，所述方法包括以下步骤：（1a）用荧光材料免疫染色目标生物材料; （1b）用另一种荧光材料在细胞膜上免疫染色另一种生物材料（参考生物材料）; （2）使用靶标和参考生物材料的免疫染色图像来鉴定对应于目标生物材料的荧光信号并测量与靶标和参照生物材料相对应的荧光信号; 和（3）通过给定的方法校正与目标生物材料相对应的荧光信号的测量值以量化表达水平。

公开(公告)号：US20160018300A1

公开(公告)日：2016-01-21

申请号：US14773148

申请日：2014-03-06

Applicant: KONICA MINOLTA, INC.

Inventor： Kensaku TAKANASHI ,  Yasushi NAKANO ,  Takeshi ISODA ,  Hideki GOUDA

IPC: G01N1/30 ,  G01N33/52

CPC classification number: G01N1/30 ,  G01N33/52 ,  G01N2001/302

Abstract: An object of the present invention is to provide: a staining agent for tissue staining which has an improved fluorescence signal evaluation accuracy; and a tissue staining kit comprising the staining agent. The staining agent for tissue staining contains, as a staining component, dye-resin particles comprising thermosetting resin particles and a fluorescent dye immobilized on the resin particles, wherein the resin particles contains a substituent having an electric charge opposite to that of the fluorescent dye and forms an ionic bond or a covalent bond with the fluorescent dye, and the dye-resin particles have a particle size variation coefficient of 15% or less.

Abstract translation: 本发明的目的是提供：具有改善的荧光信号评价精度的组织染色用染色剂; 和包含染色剂的组织染色试剂盒。 用于组织染色的染色剂包含作为染色成分的染料树脂颗粒，其包含固定在树脂颗粒上的热固性树脂颗粒和荧光染料，其中树脂颗粒含有具有与荧光染料相反的电荷的取代基， 与荧光染料形成离子键或共价键，染料树脂粒子的粒径变化系数为15％以下。

公开(公告)号：US20180172589A1

公开(公告)日：2018-06-21

申请号：US15737124

申请日：2016-06-07

Applicant: KONICA MINOLTA, INC.

Inventor： Hideki GOUDA ,  Yasuhiro WATANABI

IPC: G01N21/64 ,  G01N1/30

CPC classification number: G01N21/6428 ,  G01N1/30 ,  G01N21/64 ,  G01N33/48 ,  G01N33/582 ,  G01N33/587 ,  G01N2021/6439

Abstract: An analysis device for an objective biological substance includes a generator, a divider, an analyzer, and a calculator. The generator generates a microscopic image of a tissue sample after staining. The divider divides the microscopic image into at least one section having a prescribed size. The analyzer analyzes a staining condition of the microscopic image for each section. The calculator calculates a prescribed statistic based on an analysis result by the analyzer.

公开(公告)号：US20170342470A1

公开(公告)日：2017-11-30

申请号：US15534732

申请日：2015-12-09

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Kensaku TAKANASHI

IPC: C12Q1/68 ,  G01N21/64 ,  G01N33/58 ,  G01N33/574

CPC classification number: C12Q1/6816 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N21/64 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/53 ,  G01N33/531 ,  G01N33/533 ,  G01N33/536 ,  G01N33/54346 ,  G01N33/54393 ,  G01N33/5748 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2333/71

Abstract: [Problem ] Provided is a means for detecting and quantifying a biological substance of interest with an improved accuracy by inhibiting non-specific adsorption of fluorescent nanoparticles and thereby reducing the background noise in immunostaining with fluorescent nanoparticles. [Means for Solution] Immunostaining is carried out upon diluting fluorescent nanoparticles with a fluorescent nanoparticle diluent which contains 1 to 5% (W/W) of a protein having a molecular weight of 40,000 or higher (e,g., BSA) and 1 to 3% (W/W) of a protein having a molecular weight of less than 40,000 (eg ., casein) and, when casein is used as a low-molecular-weight protein, it is preferred that the κ-casein content in the casein is 10% (W/W) or less and the ratio of α-casein and β-casein (α-casein:β-casein) contained in the casein is 40:60 to 60:40 ( taking the total amount of α-casein and β-casein as 100).

公开(公告)号：US20170074886A1

公开(公告)日：2017-03-16

申请号：US15123578

申请日：2015-03-04

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Masaru TAKAHASHI

IPC: G01N33/58

Abstract: Provided are: a phosphor-integrated nanoparticle labeling agent which is capable of yielding a sufficient signal intensity even when its final concentration in an immunofluorescent staining reaction system is low (e.g., 0.02 nM) and enables to obtain an immunofluorescently stained image with reduced noise by inhibiting non-specific adsorption of a probe biological substance and a label to substances other than a detection subject; and an immunostaining method using the same. The phosphor-integrated nanoparticle labeling agent is a set which includes: a probe biological substance 3, which is linked to a first binding group substance A via a first hydrophilic polymer-derived spacer 1 having a length of 30 Å to 1,000 Å and specifically binds to a biomolecule 2; and a phosphor-integrated nanoparticle 5, which has a second binding group substance B capable of specifically binding to the first binding group substance A.

Abstract translation: 提供：即使在免疫荧光染色反应体系中的最终浓度低（例如，0.02nM）时也能够产生足够的信号强度并且能够获得具有降低的噪声的免疫荧光染色图像的磷光体一体化纳米颗粒标记剂 抑制探针生物物质和标签对检测对象以外的物质的非特异性吸附; 和使用其的免疫染色方法。 磷光体一体化纳米颗粒标记剂是一组，其包括：探针生物物质3，其经由长度为从Å至1000Å的第一亲水性聚合物衍生的间隔物1与第一结合基团物质A连接并特异性结合 生物分子2; 和具有能够特异性结合第一结合基物质A的第二结合基物质B的磷光体一体化纳米粒子5。

公开(公告)号：US20160178621A1

公开(公告)日：2016-06-23

申请号：US14896831

申请日：2014-04-23

Applicant: KONICA MINOLTA, INC.

Inventor： Hideki GOUDA ,  Masaru TAKAHASHI ,  Kensaku TAKANASHI ,  Fuminori OKADA

IPC: G01N33/543

CPC classification number: G01N33/54346 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  C09B67/0013 ,  C09B68/41 ,  C09B68/444

Abstract: For fluorescent nanoparticles having a zeta potential of −10 mV to −60 mV at pH 7.0 or a zeta potential of 0 mV to −10 mV in a buffer of pH 6.0 to 8.0, an appropriate electrical repulsive force can be generated between biomolecules that are generally negatively charged and the fluorescent nanoparticles. As a result, non-specific binding between the fluorescent nanoparticles and the biomolecules is surppressed and the fluorescent nanoparticles are specifically bound to a biomolecule to be stained through interaction stronger than the electrical repulsive force, so that the visibility of the specific biomolecule to be stained can be improved. Further, since an appropriate electrical repulsive force is also generated between the fluorescent nanoparticles themselves, aggregation of the fluorescent nanoparticles can be inhibited and the dispersibility in a staining solution can thereby be maintained.

Abstract translation: 对于在pH 7.0至8.0的缓冲液中，ζ电位为-10mV至-60mV或pH 6.0至8.0的缓冲液中ζ电位为0mV至-10mV的荧光纳米颗粒，可以在生物分子之间产生适当的电斥斥力 通常带负电荷和荧光纳米颗粒。 结果，荧光纳米颗粒和生物分子之间的非特异性结合被消除，并且荧光纳米颗粒被特异性结合到生物分子上，以通过相对于电排斥力更强的相互作用进行染色，使得特定生物分子的可见性被染色 可以改进。 此外，由于在荧光纳米颗粒本身之间也产生适当的电斥斥力，因此可以抑制荧光纳米粒子的聚集，从而可以保持染色溶液中的分散性。