公开(公告)号：US20180136212A1

公开(公告)日：2018-05-17

申请号：US15806970

申请日：2017-11-08

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Masaru TAKAHASHI ,  Kensaku TAKANASHI ,  Hisatake OKADA ,  Yuichi OZAKI ,  Yuka YOSHIHARA ,  Yasushi NAKANO ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE ,  Norikazu MASUDA ,  Masakazu TOI ,  Hiroshi TADA ,  Minoru MIYASHITA

IPC: G01N33/574 ,  G06K9/00

CPC classification number: G01N33/57415 ,  G01N1/30 ,  G01N33/5023 ,  G01N33/582 ,  G01N2333/71 ,  G06K9/00134 ,  G06K9/0014 ,  G06K9/00147

Abstract: The present invention relates to a test support method which is a means for predicting whether or not pathological complete response (pCR) will be attained when a preoperative chemotherapy with an anticancer drug is performed, the method using a sample (breast cancer tissue section) collected from a breast cancer patient to be subjected to the preoperative chemotherapy. The test support method includes: [1] the step of acquiring a fluorescence image of a breast cancer tissue section, which fluorescence image shows bright spots of fluorescent nanoparticles labeling one or more kinds of breast cancer-related proteins; [2] the step of acquiring at least one index relating to the expression level(s) of the breast cancer-related protein(s) on the basis of the bright spots of the fluorescence image; and [3] the step of acquiring information for predicting pCR by performing an analysis using the above-described at least one index.

公开(公告)号：US20210003486A1

公开(公告)日：2021-01-07

申请号：US17031122

申请日：2020-09-24

Applicant: Konica Minolta, Inc.

Inventor： Takuji AIMIYA ,  Hideki GOUDA ,  Hisatake OKADA ,  Yasushi NAKANO ,  Kohsuke GONDA ,  Motohiro TAKEDA ,  Noriaki OHUCHI

IPC: G01N1/30 ,  G01N33/58 ,  G01N21/64 ,  B82Y30/00 ,  B82Y15/00 ,  G01N33/74

Abstract: A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.

公开(公告)号：US20210311024A1

公开(公告)日：2021-10-07

申请号：US15999859

申请日：2017-02-15

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Takeshi SHIRAISHI ,  Takeshi ISODA ,  Noboru KOYAMA ,  Hisatake OKADA

IPC: G01N33/50 ,  G01N33/574

Abstract: An object of the present invention is to provide a non-clinical test method which allows for the quality control of experimental animals having a transplanted tumor site, such as tumor-bearing mice, or experimental animals having a lesion site other than a transplanted a tumor site, and which is characterized by including the step of identifying, using a specimen collected from such an experimental animal, the profile of a lesion site, for example, a transplanted tumor site, of the experimental animal, by a quantitative technique.

公开(公告)号：US20210041446A1

公开(公告)日：2021-02-11

申请号：US15999660

申请日：2017-02-15

Applicant: KONICA MINOLTA, INC.

Inventor： Masaru TAKAHASHI ,  Etsuko FUTAYA ,  Hisatake OKADA ,  Noboru KOYAMA

IPC: G01N33/574 ,  C12Q1/6886 ,  G01N21/64

Abstract: The present invention provides a method of obtaining information for the diagnosis or treatment, mainly of cancer or an immune system-related disease, the method including the step of quantifying the expression level of a cancer-associated protein or a nucleic acid in a tumor cell; and/or the step of quantifying the expression level of a protein or a nucleic acid in an immune cell, using a specimen derived from tumor tissue of a human or a non-human.

公开(公告)号：US20210404919A1

公开(公告)日：2021-12-30

申请号：US17469225

申请日：2021-09-08

Applicant: Konica Minolta, Inc.

Inventor： Takuji AIMIYA ,  Hideki GOUDA ,  Hisatake OKADA ,  Yasushi NAKANO ,  Kohsuke GONDA ,  Motohiro TAKEDA ,  Noriaki OHUCHI

IPC: G01N1/30 ,  G01N33/58 ,  G01N21/64 ,  B82Y30/00 ,  B82Y15/00 ,  G01N33/74

Abstract: A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.

公开(公告)号：US20180231466A1

公开(公告)日：2018-08-16

申请号：US15950648

申请日：2018-04-11

Applicant: Konica Minolta, Inc.

Inventor： Kensaku TAKANASHI ,  Hideki GODA ,  Hisatake OKADA ,  Yasushi NAKANO ,  Kohsuke GONDA ,  Motohiro TAKEDA ,  Noriaki OHUCHI

IPC: G01N21/64 ,  G01N33/483 ,  G01N33/58

CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6489 ,  G01N33/4833 ,  G01N33/582 ,  G01N33/587 ,  G01N2021/6439

Abstract: A biological substance detection method for detecting a biological substance specifically in a pathological specimen, includes a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.

公开(公告)号：US20170115300A1

公开(公告)日：2017-04-27

申请号：US15399489

申请日：2017-01-05

Applicant: Konica Minolta, Inc.

Inventor： Kensaku TAKANASHI ,  Hisatake OKADA ,  Yasushi NAKANO ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G01N33/58 ,  G01N33/574 ,  G01N21/64 ,  G01N33/569

CPC classification number: G01N33/582 ,  G01N21/6428 ,  G01N33/56966 ,  G01N33/57492 ,  G01N2021/6439 ,  G01N2333/70596

Abstract: The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of a part stained for morphological observation when the fluorescent label used for the immunostaining is observed.

公开(公告)号：US20140186857A1

公开(公告)日：2014-07-03

申请号：US14200139

申请日：2014-03-07

Applicant: Konica Minolta, Inc.

Inventor： Kensaku TAKANASHI ,  Hisatake OKADA ,  Yasushi NAKANO

IPC: G01N33/569

CPC classification number: G01N33/582 ,  G01N21/6428 ,  G01N33/56966 ,  G01N33/57492 ,  G01N2021/6439 ,  G01N2333/70596

Abstract: The present invention provides a fluorescent label that can be used for carrying out a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The fluorescent label is a fluorescent dye-containing nanoparticle in which the parent material is a cross-linked polymer and the fluorescent dye is an aromatic ring-based dye molecule. The cross-linked polymer is suitably a melamine resin or a styrene resin. The aromatic ring-based dye molecule is suitably a perylene and more suitably a perylene diimide. The dye molecule can have a polar group such as sulfonic acid group or its acid halide.

Abstract translation: 本发明提供一种荧光标记，其可用于进行用于特异性检测病理标本中的生物物质的生物物质检测方法，通过该方法，使用荧光标记进行免疫染色并使用染色剂进行形态观察染色 同时进行形态学观察，即使通过用激发光照射使荧光标记和/或染色剂劣化，也可以适当地评价荧光观察和免疫染色的结果。 荧光标记是含荧光染料的纳米颗粒，其中母体是交联聚合物，荧光染料是芳族环基染料分子。 交联聚合物适当地是三聚氰胺树脂或苯乙烯树脂。 芳族环染料分子合适地是苝，更合适的是苝二酰亚胺。 染料分子可以具有极性基团，例如磺酸基或其酰卤。