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公开(公告)号：US20150337355A1
公开(公告)日：2015-11-26
申请号：US14411191
申请日：2013-07-01
CPC分类号： C12Q1/24 , B01L3/502761 , B01L2200/027 , B01L2200/0652 , C12M25/00 , C12M33/00 , G01N15/1404 , G01N15/1459 , G01N15/1484 , G01N33/574 , G01N2015/1006 , G01N2015/1415
摘要： A cell-spreading device may include a microchamber chip having a microchamber capable of enclosing and retaining a cell, a channel-forming frame united with the microchamber chip to form a channel on the microchamber, an inlet provided in the channel-forming frame to allow a cell suspension to flow into the channel, and an outlet provided in the channel-forming frame to allow the cell suspension, which has been allowed to flow into the channel through the inlet, to flow out from the channel. When an aperture of the microchamber is projected perpendicularly to a longitudinal width of the microchamber chip, the void ratio that is a ratio of the sum total of voids to the longitudinal width is not more than 5%, the void being a length of a portion where the projected aperture of the microchamber is not present against the longitudinal width.
摘要翻译： 细胞扩散装置可以包括具有能够封闭和保持细胞的微型室的微型仓芯片，与微型仓结合在一起的通道形成框架以在微型仓上形成通道，设置在通道形成框架中以允许 流入通道的细胞悬浮液和设置在通道形成框架中的出口，以允许已经允许通过入口流入通道的细胞悬浮液从通道流出。 当微孔的孔径垂直于微孔板的纵向宽度投影时，作为空隙总和与纵向宽度的比率的空隙率不大于5％，空隙是部分的长度 其中微孔板的投影孔径不存在抵靠纵向宽度。
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公开(公告)号：US20160123851A1
公开(公告)日：2016-05-05
申请号：US14890506
申请日：2014-03-24
申请人： KONICA MINOLTA, INC.
发明人： Tsuneko CHIYODA , Jungo ARAKI
摘要： An object of the present invention is, in the case when a specific substance that has expressed on cells to be detected in a liquid sample is to be specifically stained, to provide a convenient cell staining method by which the damage and loss (flow out) of the cells in the collected sample are prevented, and the time has been shortened in a procedure for staining the cells in the liquid sample. Furthermore, another object of the present invention is to provide a sample collection tube that is suitable for use in the above-mentioned cell staining method. A method for specifically staining a specific substance possessed by cells in a sample, including performing the following steps in one step: (A) a step of performing an immobilization treatment of the cells; and (B) a step of performing a permeabilization treatment of the cells.
摘要翻译： 本发明的目的是在液体试样中要检测的细胞上表达的特定物质被特异性染色的情况下，提供方便的细胞染色方法，通过该方法损伤和流失（流出） 的收集样品中的细胞，并且在用于染色液体样品中的细胞的程序中缩短了时间。 此外，本发明的另一个目的是提供一种适用于上述细胞染色方法的样品采集管。 一种用于特异性染色样品中细胞所具有的特定物质的方法，包括在一个步骤中进行以下步骤：（A）进行细胞的固定化处理的步骤; 和（B）进行细胞透化处理的步骤。
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公开(公告)号：US20180348098A1
公开(公告)日：2018-12-06
申请号：US15770964
申请日：2016-10-25
申请人： KONICA MINOLTA, INC.
发明人： Jungo ARAKI , Manami MASUBUCHI
IPC分类号： G01N1/28
摘要： An object of the present invention is to provide a means that enables improvement of efficiency in recovery of rare cells in a cell suspension by suppressing cell adsorption to laboratory equipment but not suppressing cell adsorption to a cell spreading substrate. A cell spreading method according to the invention includes a step of adding to a cell suspension a protein in such an amount as to give a final concentration of from 0.0001% to 0.1%, and preferably from 0.0005% to 0.01% (a protein addition step) and a step of spreading cells contained in the cell suspension that has been subjected to the protein addition step on a cell spreading substrate having a contact angle with water of 60 degrees or more, and preferably from 70 to 90 degrees, and allowing to stand to cause the cells to adsorb onto the substrate (a cell spreading step).
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