公开(公告)号：US20220380732A1

公开(公告)日：2022-12-01

申请号：US17765358

申请日：2020-09-30

Applicant: KYOTO UNIVERSITY

Inventor： Kenji OSAFUNE ,  Shinichi MAE ,  Makoto RYOSAKA

IPC: C12N5/071 ,  G01N33/569

Abstract: Provided is a method for isolating a ureteric bud tip cell from cells, a tissue, or an organoid comprising the ureteric bud tip cell, comprising the following steps of contacting the cells, tissue, or organoid comprising the ureteric bud tip cell with a very low density lipoprotein receptor (VLDL-R) binding agent, and isolating the ureteric bud tip cell using the binding agent as an indicator.

公开(公告)号：US20210009960A1

公开(公告)日：2021-01-14

申请号：US16763875

申请日：2018-11-16

Applicant: Kyoto University

Inventor： Kenji OSAFUNE ,  Shinichi MAE ,  Makoto RYOUSAKA

IPC: C12N5/071

Abstract: An object of the present application is to provide a method for inducing the differentiation from pluripotent stem cells, particularly iPS cells and ES cells, into ureteric bud cells. Another object of the present application is to provide a system for producing ureteric bud-like tissue from pluripotent stem cells through each stage of differentiations, i.e. anterior primitive streak cells, anterior intermediate mesoderm cells and Wolffian duct cells. Yet another object of the present application is to provide a method for maintaining ureteric bud-like organoids. Another object of the present application is to provide a method for expansion-culturing ureteric bud-like tip tissue.