公开(公告)号：US20240010989A1

公开(公告)日：2024-01-11

申请号：US18271112

申请日：2022-01-11

Applicant: Kyoto University ,  Rege Nephro Co., Ltd.

Inventor： Kenji OSAFUNE ,  Toshikazu ARAOKA ,  Yoshifumi KOBAYASHI ,  Makoto RYOSAKA ,  Hiraku TSUJIMOTO

IPC: C12N5/071

CPC classification number: C12N5/0687 ,  C12N2506/45 ,  C12N2501/119 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/15

Abstract: A medium for culturing and expanding nephron progenitor cells, the medium containing a GSK-3β inhibitor, a ROCK inhibitor, and at least one fibroblast growth factor selected from the group consisting of FGF9 and FGF20. Also, a method for culturing and expanding nephron progenitor cells using the medium. Also, a method for producing renal organoids, the method including a step of culturing and expanding nephron progenitor cells using the above method for culturing and expanding nephron progenitor cells, and a step of differentiating the cultured and expanded nephron progenitor cells into renal organoids.

公开(公告)号：US20240110157A1

公开(公告)日：2024-04-04

申请号：US18535328

申请日：2023-12-11

Applicant: KYOTO UNIVERSITY ,  Rege Nephro Co., Ltd. ,  NIKKISO CO., LTD.

Inventor： Kenji OSAFUNE ,  Shunjiro SUGIMOTO ,  Makoto RYOSAKA ,  Yoichi JIMBO ,  Fumihiko KITAGAWA

IPC: C12N5/071

CPC classification number: C12N5/0686 ,  C12N2523/00

Abstract: There are provided a cell aggregate that can be used as a cell preparation in regenerative medicine, maintains the original characteristics of cells, and has high safety, and a method for simply and safely maintaining the cell aggregate size, the cell characteristics and viability suitable for use as a cell preparation in regenerative medicine. According to an aspect of the present invention, a cell aggregate maintaining method is a method including a maintenance process of maintaining a liquid containing a plurality of the cell aggregates at a temperature which is lower than a culture temperature of the cells and at which the liquid is not frozen.

公开(公告)号：US20220380732A1

公开(公告)日：2022-12-01

申请号：US17765358

申请日：2020-09-30

Applicant: KYOTO UNIVERSITY

Inventor： Kenji OSAFUNE ,  Shinichi MAE ,  Makoto RYOSAKA

IPC: C12N5/071 ,  G01N33/569

Abstract: Provided is a method for isolating a ureteric bud tip cell from cells, a tissue, or an organoid comprising the ureteric bud tip cell, comprising the following steps of contacting the cells, tissue, or organoid comprising the ureteric bud tip cell with a very low density lipoprotein receptor (VLDL-R) binding agent, and isolating the ureteric bud tip cell using the binding agent as an indicator.