公开(公告)号：US20140107045A1

公开(公告)日：2014-04-17

申请号：US14136347

申请日：2013-12-20

Applicant: LABORATOIRE MEDIDOM SA

Inventor： Stefano Carlino ,  René-Pierre Bunter

IPC: A61K31/7032

CPC classification number: A61K31/7032 ,  C07H15/10

Abstract: A process for preparing pure monosialoganglioside GM1 in the form of its sodium salt. There is provided a process for the isolation and purification of monosialoganglioside GM1 comprising (a) separation of GM1 from a lipidic mixture containing the monosialoganglioside GM1 as the main ganglioside component by ion exchange column-chromatography using an eluent comprising potassium or caesium ions, (b) recovery of the solute from the eluted solution, (c) diafiltration of an aqueous solution of the recovered solute, and (d) second diafiltration after the addition of 1 M NaCl, and recovering GM1. The purity level of GM1 obtained is higher than 99.0%.

Abstract translation: 一种以其钠盐形式制备纯唾液酸神经节苷脂GM1的方法。 提供了一种用于分离和纯化单唾液酸神经节苷脂GM1的方法，其包括（a）通过使用包含钾或铯离子的洗脱液的离子交换柱色谱法分离GM1从含有单唾液酸神经节苷脂GM1作为主要神经节苷脂组分的脂质混合物（b ）从洗脱的溶液中回收溶质，（c）渗滤回收的溶质的水溶液，和（d）在加入1M NaCl后进行第二次渗滤，并回收GM1。 获得的GM1纯度高于99.0％。

公开(公告)号：US09498490B2

公开(公告)日：2016-11-22

申请号：US14136347

申请日：2013-12-20

Applicant: LABORATOIRE MEDIDOM SA

Inventor： Stefano Carlino ,  René-Pierre Bunter

IPC: A61K31/7032 ,  A61P25/00 ,  C07H1/06 ,  C07H15/10

CPC classification number: A61K31/7032 ,  C07H15/10

Abstract: A process for preparing pure monosialoganglioside GM1 in the form of its sodium salt. There is provided a process for the isolation and purification of monosialoganglioside GM1 comprising (a) separation of GM1 from a lipidic mixture containing the monosialoganglioside GM1 as the main ganglioside component by ion exchange column-chromatography using an eluent comprising potassium or caesium ions, (b) recovery of the solute from the eluted solution, (c) diafiltration of an aqueous solution of the recovered solute, and (d) second diafiltration after the addition of 1 M NaCl, and recovering GM1. The purity level of GM1 obtained is higher than 99.0%.

Abstract translation: 一种以其钠盐形式制备纯唾液酸神经节苷脂GM1的方法。 提供了一种用于分离和纯化单唾液酸神经节苷脂GM1的方法，其包括（a）通过使用包含钾或铯离子的洗脱液的离子交换柱色谱法分离GM1从含有单唾液酸神经节苷脂GM1作为主要神经节苷脂组分的脂质混合物（b ）从洗脱的溶液中回收溶质，（c）渗滤回收的溶质的水溶液，和（d）在加入1M NaCl后进行第二次渗滤，并回收GM1。 获得的GM1纯度高于99.0％。

公开(公告)号：US20160375046A1

公开(公告)日：2016-12-29

申请号：US15258673

申请日：2016-09-07

Applicant: LABORATOIRE MEDIDOM SA

Inventor： Stefano Carlino ,  René-Pierre Bunter

IPC: A61K31/7032

CPC classification number: A61K31/7032 ,  C07H15/10

Abstract: A process for preparing pure monosialoganglioside GM1 in the form of its sodium salt. There is provided a process for the isolation and purification of monosialoganglioside GM1 comprising (a) separation of GM1 from a lipidic mixture containing the monosialoganglioside GM1 as the main ganglioside component by ion exchange column-chromatography using an eluent comprising potassium or caesium ions, (b) recovery of the solute from the eluted solution, (c) diafiltration of an aqueous solution of the recovered solute, and (d) second diafiltration after the addition of 1 M NaCl, and recovering GM1. The purity level of GM1 obtained is higher than 99.0%.

Abstract translation: 一种以其钠盐形式制备纯唾液酸神经节苷脂GM1的方法。 提供了用于分离和纯化单唾液酸神经节苷脂GM1的方法，其包括（a）通过使用包含钾或铯离子的洗脱液的离子交换柱色谱法分离GM1从含有单唾液酸神经节苷脂GM1作为主要神经节苷脂组分的脂质混合物（b ）从洗脱的溶液中回收溶质，（c）渗滤回收的溶质的水溶液，和（d）在加入1M NaCl后进行第二次渗滤，并回收GM1。 获得的GM1纯度高于99.0％。