公开(公告)号：US20170016811A1

公开(公告)日：2017-01-19

申请号：US15122055

申请日：2014-09-19

申请人： LG ELECTRONICS INC. ,  NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY

发明人： Chulwoo PARK ,  Sunghwa LEE ,  Akira MIZUNO ,  Kazunori TAKASHIMA ,  Hirofumi KURITA ,  Hachiro YASUDA

IPC分类号： G01N15/06 ,  G01N21/84 ,  G01N21/76 ,  C12Q1/04

CPC分类号： G01N15/0606 ,  C12Q1/04 ,  G01N15/0637 ,  G01N21/6428 ,  G01N21/76 ,  G01N21/8483 ,  G01N2015/0069 ,  G01N2021/6439

摘要： An airborne microbial measurement apparatus and a measurement method thereof are provided. An airborne microbial measurement apparatus according to an embodiment includes a discharge apparatus including a discharge electrode and a voltage supply unit applying a high voltage to the discharge electrode. A substrate is provided to a side of the discharge apparatus to collect an airborne microbe from air by a high voltage applied to the discharge electrode. A reagent injection apparatus supplies a dyeing reagent to the microbe collected on the substrate or a DNA of the microbe. A light emission measurement apparatus senses a quantity of light generated from the DNA to which the dyeing reagent is supplied. The discharge apparatus includes a controller controlling the voltage supply unit so that the voltage is applied to collect the airborne microbe or destroy an external wall of the collected airborne microbe.

摘要翻译： 提供了一种空气微生物测定装置及其测定方法。 根据实施例的空气微生物测量装置包括放电装置，其包括放电电极和向放电电极施加高电压的电压供给单元。 将基板设置在放电装置的一侧，通过施加到放电电极的高电压从空气收集空气中的微生物。 试剂注射装置将染色剂供给到收集在基底上的微生物或微生物的DNA。 发光测定装置检测从供给染色剂的DNA所产生的光量。 放电装置包括控制电压供应单元的控制器，以便施加电压以收集空气微生物或破坏收集的空气微生物的外壁。

公开(公告)号：US20150368604A1

公开(公告)日：2015-12-24

申请号：US14650560

申请日：2013-12-12

申请人： NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY

发明人： Akira MIZUNO ,  Rika NUMANO ,  Hirofumi KURITA

IPC分类号： C12M1/42 ,  C12N13/00

CPC分类号： C12M35/02 ,  C12N13/00 ,  C12N15/87

摘要： An exogenous substance transfer apparatus that introduces an exogenous substance into a small amount of cells efficiently and at low cost by electrical action and a method for manufacturing an exogenous substance-bearing cell that is able to manufacture many kinds of exogenous substance-bearing cells at low cost are provided.In the introducing of an exogenous substance such as a gene or the like into a cell, a cell suspension liquid that contains the cell and the exogenous substance is supplied to a container (2) through an opening portion (17). The cell suspension liquid is supplied to the container (2) in an amount that is prepared such that a liquid droplet (W) that is formed will not come into contact with a pair of electrodes (5a, 5b) at the same time. The liquid droplet (W) is formed in an oil (6) that is stored in the container (2), without the cell suspension liquid mixing with the oil (6). The liquid droplet (W) is disposed between the electrodes (5a, 5b). When a direct current voltage is supplied to the electrodes (5a, 5b) from a power supply (9), the liquid droplet (W) moves between the electrodes (5a, 5b), and the exogenous substance is introduced into the cell within the liquid droplet (W) by electrical action when the liquid droplet (W) comes into contact with the electrodes (5a, 5b).

摘要翻译： 一种外源性物质转移装置，其通过电作用有效且低成本地将外源性物质引入少量细胞中，以及能够制造能够低成本制造多种外源物质的细胞的外源性携带细胞的方法 提供成本。 在将诸如基因等的外源物质引入细胞中时，含有细胞和外源物质的细胞悬浮液通过开口部分（17）供给到容器（2）。 细胞悬浮液以一定量使得所形成的液滴（W）不会同时与一对电极（5a，5b）接触的量被供应到容器（2）。 在储存在容器（2）中的油（6）中形成液滴（W），而不会使液体与油（6）混合。 液滴（W）设置在电极（5a，5b）之间。 当从电源（9）向电极（5a，5b）供给直流电压时，液滴（W）在电极（5a，5b）之间移动，外源物质被引入到电池 当液滴（W）与电极（5a，5b）接触时，通过电作用产生液滴（W）。