公开(公告)号：US09434805B2

公开(公告)日：2016-09-06

申请号：US14640009

申请日：2015-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Barnett B. Rosenblum

IPC: C08F251/00 ,  C08F2/32 ,  C08F220/56 ,  C12Q1/68

CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

公开(公告)号：US09890423B2

公开(公告)日：2018-02-13

申请号：US15254193

申请日：2016-09-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Barnett B. Rosenblum

IPC: C12Q1/68 ,  C08F2/32 ,  C08F220/56 ,  C08F251/00 ,  C08F222/38

CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.