公开(公告)号：US20200149105A1

公开(公告)日：2020-05-14

申请号：US16741704

申请日：2020-01-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin LUO ,  Marina Sedova ,  David Light ,  Ryan Jones ,  Mohammad Alanjary

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: An aqueous reagent solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.

公开(公告)号：US10533220B2

公开(公告)日：2020-01-14

申请号：US15785579

申请日：2017-10-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin Luo ,  Marina Sedova ,  David Light ,  Ryan Jones ,  Mohammad Alanjary

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.

公开(公告)号：US09797011B2

公开(公告)日：2017-10-24

申请号：US14485282

申请日：2014-09-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin Luo ,  Marina Sedova ,  David Light ,  Ryan Jones ,  Mohammad Alanjary

IPC: C12Q1/68 ,  C07H21/02 ,  G01N31/22

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2563/149 ,  C12Q2563/137 ,  C12Q2535/122

Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.

公开(公告)号：US09249461B2

公开(公告)日：2016-02-02

申请号：US14044712

申请日：2013-10-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan M. Rothberg

IPC: C12Q1/68 ,  G01N27/414 ,  B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  C40B50/06

CPC classification number: B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/1093 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

公开(公告)号：US10738353B2

公开(公告)日：2020-08-11

申请号：US16120007

申请日：2018-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald Cicero ,  Christina Inman ,  Paul Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US10221451B2

公开(公告)日：2019-03-05

申请号：US14754231

申请日：2015-06-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James A. Ball

IPC: C12Q1/6874 ,  G01N27/414

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

公开(公告)号：US09932448B2

公开(公告)日：2018-04-03

申请号：US15392834

申请日：2016-12-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  Ryan Jones ,  David Light

IPC: C12N15/10 ,  C08J3/12 ,  B07B1/00

CPC classification number: C08J3/12 ,  B07B1/00 ,  C08J2300/14 ,  C12N15/1017

Abstract: A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. The filter has the retentate side and a permeate side. The method further includes, while stirring the solution within the stir cell, dispensing a buffer solution at a first flow rate to the stir cell and drawing a permeate from the permeate side of the filter using a pump at a second flow rate, the permeate including a subset of the plurality of hydrogel particles.

公开(公告)号：US09434805B2

公开(公告)日：2016-09-06

申请号：US14640009

申请日：2015-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Barnett B. Rosenblum

IPC: C08F251/00 ,  C08F2/32 ,  C08F220/56 ,  C12Q1/68

CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

公开(公告)号：US12234452B2

公开(公告)日：2025-02-25

申请号：US16841546

申请日：2020-04-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  B01J19/00 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06

Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

公开(公告)号：US11578356B2

公开(公告)日：2023-02-14

申请号：US16938192

申请日：2020-07-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald Cicero ,  Christina Inman ,  Paul Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.