公开(公告)号：US20160054327A1

公开(公告)日：2016-02-25

申请号：US14688986

申请日：2015-04-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Diller ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

公开(公告)号：US09983211B2

公开(公告)日：2018-05-29

申请号：US15138177

申请日：2016-04-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Diller ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58 ,  G01N33/68 ,  G01N33/00 ,  B33Y80/00

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

公开(公告)号：US20160341735A1

公开(公告)日：2016-11-24

申请号：US15138177

申请日：2016-04-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas DILLER ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

公开(公告)号：US20140234979A1

公开(公告)日：2014-08-21

申请号：US14079330

申请日：2013-11-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas DILLER ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

Abstract translation: 本发明提供用于在细胞裂解物或蛋白质混合物中标记，分离和分析蛋白质，特别是感兴趣的特定蛋白质的方法和组合物。 蛋白质用胺反应性或硫醇反应性荧光染料或胺反应性荧光试剂标记，其与位于蛋白质上的胺基团反应变为荧光。 在标记步骤之后，可以分离和分析混合物内的蛋白质。 在另一个实施方案中，加入可结合标记蛋白上的标签的标签结合荧光试剂以特异性标记所关注的蛋白质。