公开(公告)号：USD822052S1

公开(公告)日：2018-07-03

申请号：US29618478

申请日：2017-09-21

Applicant: Life Technologies Corporation

Designer： Scott Rickes ,  Laurel Stone ,  Kyle Curts ,  George Hanson ,  Adam Zahner ,  Lubasha Chabansky ,  Zenia Wei

公开(公告)号：USD951283S1

公开(公告)日：2022-05-10

申请号：US29631068

申请日：2017-12-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Laurel Stone ,  George Hanson ,  Zenia Wei ,  Kim Ippolito

公开(公告)号：US09983211B2

公开(公告)日：2018-05-29

申请号：US15138177

申请日：2016-04-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Diller ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58 ,  G01N33/68 ,  G01N33/00 ,  B33Y80/00

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

公开(公告)号：US20160054327A1

公开(公告)日：2016-02-25

申请号：US14688986

申请日：2015-04-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Diller ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

公开(公告)号：USD851119S1

公开(公告)日：2019-06-11

申请号：US29650050

申请日：2018-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Scott Rickes ,  Laurel Stone ,  Kyle Curts ,  George Hanson ,  Adam Zahner ,  Lubasha Chabansky ,  Zenia Wei

公开(公告)号：USD798309S1

公开(公告)日：2017-09-26

申请号：US29496669

申请日：2014-07-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Scott Rickes ,  Laurel Stone ,  Kyle Curts ,  George Hanson ,  Adam Zahner ,  Lubasha Chabansky ,  Zenia Wei

公开(公告)号：USD894943S1

公开(公告)日：2020-09-01

申请号：US29693243

申请日：2019-05-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Scott Rickes ,  Laurel Stone ,  Kyle Curts ,  George Hanson ,  Adam Zahner ,  Lubasha Chabansky ,  Zenia Wei

公开(公告)号：US20160341735A1

公开(公告)日：2016-11-24

申请号：US15138177

申请日：2016-04-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas DILLER ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

公开(公告)号：US20160010113A1

公开(公告)日：2016-01-14

申请号：US14801757

申请日：2015-07-16

Applicant: Life Technologies Corporation

Inventor： Uma LAKSHMIPATHY ,  Bhaskar Thyagarajan ,  Jonathan Chesnut ,  Vasiliki Anest ,  Robert Bennett ,  Pauline Lieu ,  George Hanson ,  David Thompson ,  Lucas Chase ,  Gary Shipley ,  Elizabeth Wilson

IPC: C12N15/86

CPC classification number: C12N15/86 ,  C12N2710/16221 ,  C12N2710/16222 ,  C12N2710/16243 ,  C12N2799/022 ,  C12N2799/026 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2820/002 ,  C12N2820/007 ,  C12N2820/60

Abstract: The disclosure relates generally to genetic manipulation of stem and primary cells and to reprogramming of somatic cells, more specifically, human cells. In particular, compositions and methods are disclosed for the generation and maintenance of such engineered cells.

Abstract translation: 本公开一般涉及干细胞和原代细胞的遗传操作和体细胞，更具体地，人细胞的重编程。 特别地，公开了用于产生和维护这种工程化细胞的组合物和方法。

公开(公告)号：US20140234979A1

公开(公告)日：2014-08-21

申请号：US14079330

申请日：2013-11-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas DILLER ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

Abstract translation: 本发明提供用于在细胞裂解物或蛋白质混合物中标记，分离和分析蛋白质，特别是感兴趣的特定蛋白质的方法和组合物。 蛋白质用胺反应性或硫醇反应性荧光染料或胺反应性荧光试剂标记，其与位于蛋白质上的胺基团反应变为荧光。 在标记步骤之后，可以分离和分析混合物内的蛋白质。 在另一个实施方案中，加入可结合标记蛋白上的标签的标签结合荧光试剂以特异性标记所关注的蛋白质。