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    Apparatus for and Method of Processing Biological Samples
    3.
    发明申请
    Apparatus for and Method of Processing Biological Samples 有权
    用于处理生物样品的装置和方法

    公开(公告)号：US20150044758A1

    公开(公告)日：2015-02-12

    申请号：US14462640

    申请日：2014-08-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

    IPC: B01L3/00

    CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L7/00 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  G01N2035/00306 ,  G01N2035/1037 ,  Y10T436/2575

    Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

    Abstract translation: 本发明提供了用于自动化生物处理的系统，装置，装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于免疫沉淀，染色质免疫沉淀，重组蛋白分离，核酸分离和分离，蛋白质标记，分离和分离，细胞分离和分离，食品安全分析和自动珠 基于分离。 在一些实施方案中，本发明提供自动化系统，自动化装置，自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统，自动化装置，自动化盒和用于分离，制备和纯化核酸例如DNA或RNA或其片段的自动化方法，包括质粒DNA，基因组DNA，细菌DNA，病毒DNA和任何其它DNA， 以及用于自动化系统，自动化设备，自动化盒和用于处理，分离和纯化蛋白质，肽等的自动化盒和自动化方法。

    Apparatus for and method of processing biological samples
    5.
    发明授权
    Apparatus for and method of processing biological samples 有权

    公开(公告)号：US11235323B2

    公开(公告)日：2022-02-01

    申请号：US16557192

    申请日：2019-08-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

    IPC: B01L1/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00 ,  G01N35/10

    Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

    RAPID PROTEIN LABELING AND ANALYSIS
    7.
    发明申请
    RAPID PROTEIN LABELING AND ANALYSIS 有权

    公开(公告)号：US20160341735A1

    公开(公告)日：2016-11-24

    申请号：US15138177

    申请日：2016-04-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Thomas DILLER ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

    IPC: G01N33/58

    CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

    Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

    Rapid Protein Labeling and Analysis
    8.
    发明申请
    Rapid Protein Labeling and Analysis 审中-公开
    快速蛋白质标记和分析

    公开(公告)号：US20140234979A1

    公开(公告)日：2014-08-21

    申请号：US14079330

    申请日：2013-11-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Thomas DILLER ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

    IPC: G01N33/58

    CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

    Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

    Abstract translation: 本发明提供用于在细胞裂解物或蛋白质混合物中标记，分离和分析蛋白质，特别是感兴趣的特定蛋白质的方法和组合物。 蛋白质用胺反应性或硫醇反应性荧光染料或胺反应性荧光试剂标记，其与位于蛋白质上的胺基团反应变为荧光。 在标记步骤之后，可以分离和分析混合物内的蛋白质。 在另一个实施方案中，加入可结合标记蛋白上的标签的标签结合荧光试剂以特异性标记所关注的蛋白质。

    System for rapid high-resolution gel electrophoresis
    9.
    发明授权
    System for rapid high-resolution gel electrophoresis 有权

    公开(公告)号：US11105769B2

    公开(公告)日：2021-08-31

    申请号：US16551364

    申请日：2019-08-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Timothy Updyke ,  Jennifer Miller

    IPC: G01N27/447 ,  G01N33/561

    Abstract: Electrophoretic systems, formulations and methods are described which allow a user to perform electrophoresis experiments under conditions of high voltage and with reduced run time. An electrophoretic system, formulation or method may be run at 50% higher field strength than comparable systems already in use in the art. The presently described systems and formulations may be run at voltages above 225 V, above 250 V, above 275 V, above 300 V, above 325 V or above 350 V. The time required for performing an electrophoresis experiment may be reduced to less than about 30 minutes, less than about 20 minutes, less than about 15 minutes or less than about 12 minutes.

    Apparatus for and method of processing biological samples
    10.
    发明授权
    Apparatus for and method of processing biological samples 有权

    公开(公告)号：US10434509B2

    公开(公告)日：2019-10-08

    申请号：US15719130

    申请日：2017-09-28

    Applicant: Life Technologies Corporation

    Inventor： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

    IPC: G01N1/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00 ,  G01N35/10

    Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

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