公开(公告)号：US11215803B2

公开(公告)日：2022-01-04

申请号：US15825895

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Lytle ,  Paul Boeschoten ,  Andrew Gunderson ,  Chris Gnehm

IPC: G02B21/00 ,  G02B21/36 ,  G02B27/14

Abstract: An apparatus for generating structured illumination images, comprising a housing; imaging optics mounted within the housing, the imaging optics configured to focus illumination from an illumination source such that an in-focus pattern of light corresponding to a pattern on a photomask is projected onto a sample; a reflector mounted within the housing and configured to reflect the illumination to a sample; a filter apparatus, comprising one or more filters mounted within the housing, the filter apparatus configured to allow only emissions from the illuminated sample to pass through to the sensor; and mounting features on the housing, the mounting features configured to allow the apparatus to be installed within an imaging system.

公开(公告)号：US10782514B2

公开(公告)日：2020-09-22

申请号：US15826125

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Lytle ,  Paul Boeschoten ,  Andrew Gunderson ,  Larry Rystrom ,  Chris Gnehm

IPC: G02B21/00 ,  G01N21/27 ,  G01N21/64

Abstract: A method for calibrating an imaging system can include at least the following method acts: illuminating a sample through a pinhole mask using an excitation light; capturing an image of the sample using a sensor; converting the image into data; in a processing module: filtering the data using a known spacing of pinholes in the pinhole mask to obtain filtered data that corresponds to the known spacing, using a threshold to identify regions of the filtered data that are bright enough to be associated with a pinhole, calculating the centroids of the regions, and fitting a known pattern for the pinhole mask to the regions in order to identify the best fit data for the filtered data; and storing, in a storage medium, the best fit data for use in a subsequent confocal capture routine.