公开(公告)号：US11215803B2

公开(公告)日：2022-01-04

申请号：US15825895

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Lytle ,  Paul Boeschoten ,  Andrew Gunderson ,  Chris Gnehm

IPC: G02B21/00 ,  G02B21/36 ,  G02B27/14

Abstract: An apparatus for generating structured illumination images, comprising a housing; imaging optics mounted within the housing, the imaging optics configured to focus illumination from an illumination source such that an in-focus pattern of light corresponding to a pattern on a photomask is projected onto a sample; a reflector mounted within the housing and configured to reflect the illumination to a sample; a filter apparatus, comprising one or more filters mounted within the housing, the filter apparatus configured to allow only emissions from the illuminated sample to pass through to the sensor; and mounting features on the housing, the mounting features configured to allow the apparatus to be installed within an imaging system.

公开(公告)号：USD777195S1

公开(公告)日：2017-01-24

申请号：US29543099

申请日：2015-10-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Robert Dain ,  Terence Tam ,  Steven Lytle ,  Paul Boeschoten ,  Larry Rystrom ,  Jonathan Paullin

公开(公告)号：US10782514B2

公开(公告)日：2020-09-22

申请号：US15826125

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Lytle ,  Paul Boeschoten ,  Andrew Gunderson ,  Larry Rystrom ,  Chris Gnehm

IPC: G02B21/00 ,  G01N21/27 ,  G01N21/64

Abstract: A method for calibrating an imaging system can include at least the following method acts: illuminating a sample through a pinhole mask using an excitation light; capturing an image of the sample using a sensor; converting the image into data; in a processing module: filtering the data using a known spacing of pinholes in the pinhole mask to obtain filtered data that corresponds to the known spacing, using a threshold to identify regions of the filtered data that are bright enough to be associated with a pinhole, calculating the centroids of the regions, and fitting a known pattern for the pinhole mask to the regions in order to identify the best fit data for the filtered data; and storing, in a storage medium, the best fit data for use in a subsequent confocal capture routine.

公开(公告)号：US20140340499A1

公开(公告)日：2014-11-20

申请号：US13894185

申请日：2013-05-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： TERENCE TAM ,  Frank Metting ,  Jason Mecham ,  Robert Dain ,  Larry Rystrom ,  Christopher Martin ,  Paul Boeschoten ,  Steven Lytle

IPC: G02B21/36

CPC classification number: G02B21/365

Abstract: Embodiments relate to systems and methods for sample image capture using integrated control. A digital microscope or other imaging device can be associated with a sample chamber containing cell, tissue, or other sample material. The chamber can be configured to operate using a variety of environmental variables, including gas concentration, temperature, humidity, and others. The imaging device can be configured to operate using a variety of imaging variables, including magnification, focal length, illumination, and others. A central system control module can be used to configure the settings of those hardware elements, as well as others, to set up and carry out an image capture event. The system control module can be operated to control the physical, optical, chemical, and/or other parameters of the overall imaging environment from one central control point. The variables used to produce the image capture can be configured to dynamically variable during the media capture event.

Abstract translation: 实施例涉及使用集成控制的样本图像捕获的系统和方法。 数字显微镜或其他成像装置可以与包含细胞，组织或其它样品材料的样品室相关联。 该室可以配置为使用各种环境变量进行操作，包括气体浓度，温度，湿度等。 成像装置可以被配置为使用各种成像变量进行操作，包括放大倍数，焦距，照明等。 可以使用中央系统控制模块来配置这些硬件元件以及其他硬件元件的设置，以设置和执行图像捕获事件。 可以操作系统控制模块以从一个中央控制点控制整个成像环境的物理，光学，化学和/或其他参数。 用于产生图像捕获的变量可以配置为在媒体捕获事件期间动态变化。