公开(公告)号：US10597705B2

公开(公告)日：2020-03-24

申请号：US16154529

申请日：2018-10-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12P19/34 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/46

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US20160186224A1

公开(公告)日：2016-06-30

申请号：US15071086

申请日：2016-03-15

Applicant: Life Technologies Corporation

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12P19/34

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US10093969B2

公开(公告)日：2018-10-09

申请号：US15695244

申请日：2017-09-05

Applicant: Life Technologies Corporation

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12Q1/68 ,  C12Q1/6853 ,  C12P19/34 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/46

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US09315861B2

公开(公告)日：2016-04-19

申请号：US14360892

申请日：2012-11-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract translation: 在一些实施方案中，用于连接核酸末端的方法包括：在存在可产生可连接的末端5'磷酸基团的至少一种试剂的存在下进行核酸连接反应，通过从核酸的末端5'磷酸脱除腺苷酸基 。 在一些实施方案中，阿伐他汀酶可以催化从核酸的末端5'磷酸脱除腺苷酸基团。 在一些实施方案中，用于连接核酸末端的方法包括：在适于连接核酸末端的条件下，在阿伐他汀酶存在下进行核酸连接反应。

公开(公告)号：US09765388B2

公开(公告)日：2017-09-19

申请号：US15071086

申请日：2016-03-15

Applicant: Life Technologies Corporation

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US20150031026A1

公开(公告)日：2015-01-29

申请号：US14360892

申请日：2012-11-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract translation: 在一些实施方案中，用于连接核酸末端的方法包括：在存在可产生可连接的末端5'磷酸基团的至少一种试剂的存在下进行核酸连接反应，通过从核酸的末端5'磷酸脱除腺苷酸基 。 在一些实施方案中，阿伐他汀酶可以催化从核酸的末端5'磷酸脱除腺苷酸基团。 在一些实施方案中，用于连接核酸末端的方法包括：在适于连接核酸末端的条件下，在阿伐他汀酶存在下进行核酸连接反应。