公开(公告)号：US20190170680A1

公开(公告)日：2019-06-06

申请号：US16196502

申请日：2018-11-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marcin Sikora ,  Melville Davey ,  Christian Koller ,  Simon Cawley ,  Alan Williams ,  David Kulp

IPC: G01N27/414 ,  G16B30/00

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

公开(公告)号：US11474070B2

公开(公告)日：2022-10-18

申请号：US16196502

申请日：2018-11-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marcin Sikora ,  Melville Davey ,  Christian Koller ,  Simon Cawley ,  Alan Williams ,  David Kulp

IPC: G01N27/414 ,  G16B30/00 ,  G16B30/10 ,  C12Q1/6869 ,  G01N27/27 ,  G16B30/20

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.