公开(公告)号：US20160076095A1

公开(公告)日：2016-03-17

申请号：US14856614

申请日：2015-09-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Min-Yi SHEN ,  Douglas GREINER

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01J2219/005 ,  C12Q1/6874 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2545/101 ,  G01N2201/1242 ,  G16B30/00 ,  C12Q2563/149

Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.

Abstract translation: 公开了一种用于进行核酸样品测序的质量控制的系统。 该系统包含一组固体支持物，每个固体支持物附着有多个核酸序列，其中该组包含多组固体支持物，并且每组含有与其相连的相同核酸序列的固体支持物。 各组的核酸序列彼此不同。 核酸序列是合成衍生的，并且设计核酸序列使得核酸序列在测序运行期间产生可检测信号的预定模式。 还公开了制备用于进行核酸样品测序的质量控制的方法，在核酸测序实验期间验证核酸测序仪的方法，以及在核酸测序实验期间处理核酸测序数据的方法。