公开(公告)号：US20160108381A1

公开(公告)日：2016-04-21

申请号：US14875576

申请日：2015-10-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jun LEE ,  Gary Gerard ,  Harini Shandilya ,  Katherine Griffiths ,  Moreland Gibbs ,  Peter Bergquist ,  Robert Potter

IPC: C12N9/12 ,  C12N9/14 ,  C12P19/34

CPC classification number: C12N9/1252 ,  C12N9/1276 ,  C12N9/14 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Y207/07007 ,  C12Y306/01 ,  C12Q2521/101

Abstract: The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.

Abstract translation: 本发明提供具有核苷酸聚合酶活性的多肽和增强聚合酶活性的方法。 本发明的多肽可以具有DNA依赖性DNA聚合酶活性和RNA依赖性DNA聚合酶活性，即逆转录酶活性。 本发明的多肽可用于任何应用，包括但不限于DNA测序反应，扩增反应，cDNA合成反应以及合并的cDNA合成和扩增反应，例如RT-PCR。