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公开(公告)号：US10428327B2
公开(公告)日：2019-10-01
申请号：US15605586
申请日：2017-05-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xiquan Liang , Robert Potter , Namritha Ravinder
IPC: C12N15/10 , C12N15/113 , C12N15/90
Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.
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公开(公告)号：US20160108381A1
公开(公告)日：2016-04-21
申请号：US14875576
申请日：2015-10-05
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jun LEE , Gary Gerard , Harini Shandilya , Katherine Griffiths , Moreland Gibbs , Peter Bergquist , Robert Potter
CPC classification number: C12N9/1252 , C12N9/1276 , C12N9/14 , C12P19/34 , C12Q1/6834 , C12Q1/6869 , C12Y207/07007 , C12Y306/01 , C12Q2521/101
Abstract: The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.
Abstract translation: 本发明提供具有核苷酸聚合酶活性的多肽和增强聚合酶活性的方法。 本发明的多肽可以具有DNA依赖性DNA聚合酶活性和RNA依赖性DNA聚合酶活性，即逆转录酶活性。 本发明的多肽可用于任何应用，包括但不限于DNA测序反应，扩增反应，cDNA合成反应以及合并的cDNA合成和扩增反应，例如RT-PCR。
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Patent Agency Ranking
公开(公告)号：US20160272955A1
公开(公告)日：2016-09-22
申请号：US15173439
申请日：2016-06-03
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Weidong ZHENG , Jun Lee , Robert Potter , David Mandelman
CPC classification number: C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6806 , C12Q1/686 , C12Y207/07007 , Y02P20/52 , C12Q2561/101 , C12Q2527/149 , C12Q2521/101
Abstract: A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis.
Abstract translation: 一种热稳定DNA聚合酶组合物，其包含至少两种DNA聚合酶，其中之一基本上降低了5'-核酸外切酶活性，其中一种具有5'-核酸外切酶活性。 该聚合酶可用于包括但不限于核酸合成，DNA测序，核酸扩增和cDNA合成的方法。
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公开(公告)号：US20140377810A1
公开(公告)日：2014-12-25
申请号：US14450925
申请日：2014-08-04
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jun LEE , Robert Potter , David Mandelman
CPC classification number: C12Q1/6806 , C12N9/1252 , C12N9/1276 , C12N15/62 , C12Q1/6848 , C12Q2522/101
Abstract: Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
Abstract translation: 包含单链DNA结合蛋白和核酸聚合酶（例如DNA聚合酶或逆转录酶）的融合蛋白。 这些高保真蛋白质适用于核酸扩增方法，包括聚合酶链反应（PCR）。
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公开(公告)号：US09879283B2
公开(公告)日：2018-01-30
申请号：US14879872
申请日：2015-10-09
Inventor： Namritha Ravinder , Korbinian Heil , Yizhu Guo , Xiquan Liang , Robert Potter , Sanjay Kumar
CPC classification number: C12N15/907 , C12N15/102 , C12N15/11 , C12N2310/3519 , C12P19/34 , C12Q1/686 , C12Q2525/143
Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.
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公开(公告)号：US09580743B2
公开(公告)日：2017-02-28
申请号：US14450925
申请日：2014-08-04
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jun Lee , Robert Potter , David Mandelman
CPC classification number: C12Q1/6806 , C12N9/1252 , C12N9/1276 , C12N15/62 , C12Q1/6848 , C12Q2522/101
Abstract: Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
Abstract translation: 包含单链DNA结合蛋白和核酸聚合酶（例如DNA聚合酶或逆转录酶）的融合蛋白。 这些高保真蛋白质适用于核酸扩增方法，包括聚合酶链反应（PCR）。
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公开(公告)号：US20160102322A1
公开(公告)日：2016-04-14
申请号：US14879872
申请日：2015-10-09
Inventor： Namritha Ravinder , Korbinian Heil , Yizhu Guo , Xiquan Liang , Robert Potter
CPC classification number: C12N15/907 , C12N15/102 , C12N15/11 , C12N2310/3519 , C12P19/34 , C12Q1/686 , C12Q2525/143
Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.
Abstract translation: 本公开一般涉及用于细胞遗传修饰的组合物和方法。 特别地，本公开涉及CRISPR试剂和这些试剂的用途。
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