公开(公告)号：US20160326515A1

公开(公告)日：2016-11-10

申请号：US15216642

申请日：2016-07-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim BREVNOV ,  Hemant PAWAR ,  Manohar FURTADO ,  Jaiprakash SHEWALE

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

公开(公告)号：US20140039176A1

公开(公告)日：2014-02-06

申请号：US13931110

申请日：2013-06-28

Applicant: Life Technologies Corporation

Inventor： Maxim BREVNOV ,  Hemant PAWAR ,  Manohar FURTADO ,  Jaiprakash SHEWALE

IPC: C12N15/10

CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

Abstract translation: 公开了用于从溶液中回收生物聚合物的方法和材料。 特别地，本发明提供从生物材料中提取和分离核酸的方法。 在洗涤剂和溶剂的存在下，可以通过与可溶性多糖聚合物和磁性颗粒形成稳定的络合物来分离核酸。 当颗粒从溶液中磁性分离出来时，核酸与它们分离。 随后可以将核酸从颗粒中释放并分离出来。 核酸制备物可用于实现下游分子技术的有效和准确的结果，例如定量，核酸来源的鉴定和基因分型。