公开(公告)号：US20220088561A1

公开(公告)日：2022-03-24

申请号：US17505075

申请日：2021-10-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Colin BRENAN ,  Jamie CHO ,  Javier GARCIA ,  Robert HESS ,  Tanya KANIGAN ,  Arrin KATZ ,  Namyong KIM ,  John LINTON ,  Shailesh SRIVASTAVA ,  Karl YODER

IPC: B01J19/00 ,  B01L3/00

Abstract: A method of registering a location of a dispenser array in relation to a microfluidic array is provided. One of the dispenser array and the microfluidic array can be movable in relation to the frame, and the other can be fixed relative to the frame. Their relative positions can be identified by a set of coordinates. Identification of a fiducial marker can occur in a manner permitting the fiducial reference to appear in a first position of a field of view of a first camera when the dispenser array or the microfluidic array is in an alignment position. Quantities related to a vector displacement from the alignment position to a fixed position on the microfluidic array or the dispenser array can be identified. Quantities determined can be used to guide positioning of the dispenser array relative to the microfluidic array.

公开(公告)号：US20200055016A1

公开(公告)日：2020-02-20

申请号：US16284953

申请日：2019-02-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Colin BRENAN ,  Jamie CHO ,  Javier GARCIA ,  Robert HESS ,  Tanya Kanigan ,  Arrin Katz ,  Namyong KIM ,  John LINTON ,  Shailesh SRIVASTAVA ,  Karl Yoder

IPC: B01J19/00 ,  B01L3/00

Abstract: A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one exterior surface that is selectively coated with an optionally dissolvable coating agent physisorbed to at least one interior surface, wherein the optionally dissolvable coating agent comprises a coating agent and a first component for the plurality of specified reactions. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. A first one of the dispenser array and the microfluidic array is movable in relation to the frame, and the other of the first one of the dispenser array and the microfluidic array is fixed relative to the frame. Quantities related to a vector displacement from the alignment position to a fixed position on the one of the dispenser array and the microfluidic array is determined. The quantities thus determined are used to guide positioning of the dispenser array relative to the microfluidic array.