公开(公告)号：US20160332133A1

公开(公告)日：2016-11-17

申请号：US15131101

申请日：2016-04-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert HESS ,  John LINTON ,  Tanya S. KANIGAN ,  Colin BRENAN ,  Can OZBAL

IPC: B01J19/00 ,  B01L3/02 ,  G01N30/46 ,  C40B60/14 ,  G01N30/60 ,  B01L3/00 ,  C12M1/32

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00351 ,  B01J2219/0036 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00389 ,  B01J2219/00423 ,  B01J2219/0043 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00673 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5085 ,  B01L3/50857 ,  B01L99/00 ,  B01L2200/0657 ,  B01L2300/0845 ,  C12M23/12 ,  C40B60/14 ,  G01N30/466 ,  G01N30/6091 ,  G01N30/6095 ,  G01N30/82 ,  G01N2030/8417

Abstract: The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

Abstract translation: 本发明的特征在于制造具有高密度阵列通孔的装置或“压板”的方法，以及清洁和翻新压板表面的方法。 本发明还涉及制备化学，生物化学和生物化合物的高密度阵列的方法，其具有优于常规的较低密度阵列的许多优点。 本发明包括许多物理，化学或生物转化可以在装置的每个可寻址通孔内串联或并行地实现的方法。 此外，本发明包括分析阵列的内容物的方法，包括测定样品的物理性质。

公开(公告)号：US20220088561A1

公开(公告)日：2022-03-24

申请号：US17505075

申请日：2021-10-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Colin BRENAN ,  Jamie CHO ,  Javier GARCIA ,  Robert HESS ,  Tanya KANIGAN ,  Arrin KATZ ,  Namyong KIM ,  John LINTON ,  Shailesh SRIVASTAVA ,  Karl YODER

IPC: B01J19/00 ,  B01L3/00

Abstract: A method of registering a location of a dispenser array in relation to a microfluidic array is provided. One of the dispenser array and the microfluidic array can be movable in relation to the frame, and the other can be fixed relative to the frame. Their relative positions can be identified by a set of coordinates. Identification of a fiducial marker can occur in a manner permitting the fiducial reference to appear in a first position of a field of view of a first camera when the dispenser array or the microfluidic array is in an alignment position. Quantities related to a vector displacement from the alignment position to a fixed position on the microfluidic array or the dispenser array can be identified. Quantities determined can be used to guide positioning of the dispenser array relative to the microfluidic array.

公开(公告)号：US20200055016A1

公开(公告)日：2020-02-20

申请号：US16284953

申请日：2019-02-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Colin BRENAN ,  Jamie CHO ,  Javier GARCIA ,  Robert HESS ,  Tanya Kanigan ,  Arrin Katz ,  Namyong KIM ,  John LINTON ,  Shailesh SRIVASTAVA ,  Karl Yoder

IPC: B01J19/00 ,  B01L3/00

Abstract: A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one exterior surface that is selectively coated with an optionally dissolvable coating agent physisorbed to at least one interior surface, wherein the optionally dissolvable coating agent comprises a coating agent and a first component for the plurality of specified reactions. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. A first one of the dispenser array and the microfluidic array is movable in relation to the frame, and the other of the first one of the dispenser array and the microfluidic array is fixed relative to the frame. Quantities related to a vector displacement from the alignment position to a fixed position on the one of the dispenser array and the microfluidic array is determined. The quantities thus determined are used to guide positioning of the dispenser array relative to the microfluidic array.

公开(公告)号：US20190118173A1

公开(公告)日：2019-04-25

申请号：US16119327

申请日：2018-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tanya S. KANIGAN ,  Steve SMITH ,  John LINTON ,  Robert HESS ,  Karl YODER ,  Colin J. H. BRENAN

IPC: B01L3/00 ,  B01L3/02

Abstract: An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

公开(公告)号：US20160167044A1

公开(公告)日：2016-06-16

申请号：US15049710

申请日：2016-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tanya S. KANIGAN ,  Steve SMITH ,  John LINTON ,  Robert HESS ,  Karl YODER ,  Colin John Herbert BRENAN

IPC: B01L3/00

CPC classification number: B01L3/5085 ,  B01L3/0241 ,  B01L3/0244 ,  B01L3/5025 ,  B01L3/50857 ,  B01L3/563 ,  B01L2200/021 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0636 ,  B01L2200/0642 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/12 ,  B01L2300/161 ,  B33Y70/00 ,  B33Y80/00 ,  Y10T137/0318 ,  Y10T137/1624 ,  Y10T137/206 ,  Y10T436/2525

Abstract: An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

Abstract translation: 提供了用于将微流体样品存储在纳升级样品芯片中的界面。 流体通道结构从样品阱阵列向选定的样品阱子集提供流体通路区域。 流体引入机构将样品流体引入流体存取区域，使得所选择的子集中的样品孔填充有样品流体，而未选择的样品池填充有样品流体。