公开(公告)号：US20140378330A1

公开(公告)日：2014-12-25

申请号：US14314985

申请日：2014-06-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Olga PETRAUSKENE ,  Pius Brzoska ,  Somaya Bit ,  Jen-Kuei Liu ,  Robert Tebbs ,  Manohar Furtado

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.

Abstract translation: 公开了一种多引物扩增测定法，用于利用在单个容器中与样品接触的多个寡核苷酸引物检测支原体物种和密切相关物种的检测扩增产物的方法和试剂盒，其中扩增产物的存在表示支原体 在样品中。

公开(公告)号：US11619608B2

公开(公告)日：2023-04-04

申请号：US16615804

申请日：2018-05-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  INTEGENX INC.

Inventor： Jen-Kuei Liu ,  Bharti Solanki ,  Hans Michael Wenz ,  Frank Mercer ,  Cevat Akin ,  Achim Karger ,  Annelise Barron ,  Scott Mack

IPC: C02F1/40 ,  C02F11/00 ,  C08G61/04 ,  G01N27/447 ,  C08L33/26

Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.

公开(公告)号：US12153014B2

公开(公告)日：2024-11-26

申请号：US18108471

申请日：2023-02-10

Applicant: LIFE TECHNOLOGIES CORPORATION ,  INTEGENX INC.

Inventor： Jen-Kuei Liu ,  Bharti Solanki ,  Hans Michael Wenz ,  Frank Mercer ,  Cevat Akin ,  Achim Karger ,  Annelise Barron ,  Scott Mack

IPC: G01N27/447 ,  C08L33/26

Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.

公开(公告)号：US09850545B2

公开(公告)日：2017-12-26

申请号：US14314985

申请日：2014-06-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Olga Petrauskene ,  Pius Brzoska ,  Somaya Bit ,  Jen-Kuei Liu ,  Robert Tebbs ,  Manohar Furtado

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.