公开(公告)号：US11453917B2

公开(公告)日：2022-09-27

申请号：US16840168

申请日：2020-04-03

Applicant: Life Technologies Corporation

Inventor： Rixun Fang ,  Manohar Furtado ,  Manfred Kayser ,  Kaye Ballantyne

IPC: C12Q1/6888

Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

公开(公告)号：US20200299786A1

公开(公告)日：2020-09-24

申请号：US16840168

申请日：2020-04-03

Applicant: Life Technologies Corporation

Inventor： Rixun Fang ,  Manohar Furtado ,  Manfred Kayser ,  Kaye Ballantyne

IPC: C12Q1/6888

Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

公开(公告)号：US20150267264A1

公开(公告)日：2015-09-24

申请号：US14624185

申请日：2015-02-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Rixun FANG ,  Manohar Furtado ,  Manfred Kayser ,  Kaye Ballantyne

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172

Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

Abstract translation: 本文提供的方法和组合物涉及在人类Y染色体上发现的13种STR标记，与目前已知的173种其它Y-STR标记物相比具有惊人的高突变率。 RM-Y-STR的集合可以克服由于其非凡的突变特性而导致的法医应用中Y染色体分析目前的困境。 本发明的实施方案包括用于等位基因测定快速突变的Y-STR标记的方法，用于分析快速突变的Y-STR标记的扩增引物，用于分析快速突变Y-STR标记的等位基因梯度和用于分析的试剂盒 的快速突变的Y-STR标记。