公开(公告)号：US12129461B2

公开(公告)日：2024-10-29

申请号：US17181103

申请日：2021-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim G. Brevnov ,  Hemant S. Pawar ,  Manohar R. Furtado ,  Jaiprakash G. Shewale

IPC: C12Q1/6806 ,  C12N15/10

CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

公开(公告)号：US10961528B2

公开(公告)日：2021-03-30

申请号：US16011597

申请日：2018-06-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim G. Brevnov ,  Hemant S. Pawar ,  Manohar R. Furtado ,  Jaiprakash G. Shewale

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.