公开(公告)号：US20180362962A1

公开(公告)日：2018-12-20

申请号：US16011597

申请日：2018-06-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim G. BREVNOV ,  Hemant S. PAWAR ,  Manohar R. FURTADO ,  Jaiprakash G. SHEWALE

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

公开(公告)号：US20210246438A1

公开(公告)日：2021-08-12

申请号：US17181103

申请日：2021-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim G. BREVNOV ,  Hemant S. PAWAR ,  Manohar R. FURTADO ,  Jaiprakash G. SHEWALE

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

公开(公告)号：US20150225774A1

公开(公告)日：2015-08-13

申请号：US14603076

申请日：2015-01-22

Applicant: Life Technologies Corporation

Inventor： Maxim G. BREVNOV ,  Elena BOLCHAKOVA ,  Manohar FURTADO

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6869 ,  C12Q2563/131 ,  C12Q2563/143

Abstract: A method for preparing a nucleic acid sample for nucleic acid sequencing includes amplifying a nucleic acid target sequence using a primer bound to a first capture substrate; capturing an amplified nucleic acid product by the first capture substrate; generating at least one sequencing ladder from the amplified nucleic acid product using at least one sequencing primer; capturing the at least one sequencing ladder by hybridizing the at least one sequencing ladder to a complementary capture compound on a second capture substrate; and removing the at least one sequencing ladder from the second capture substrate. The first and/or second capture substrate may include a magnetic particle. Other methods, workflows, kits, and computer program media for nucleic acid sample preparation are also disclosed.

Abstract translation: 用于制备用于核酸测序的核酸样品的方法包括使用与第一捕获底物结合的引物扩增核酸靶序列; 通过第一捕获基质捕获扩增的核酸产物; 使用至少一个测序引物从扩增的核酸产物产生至少一个测序梯; 通过将所述至少一个测序梯度杂交到第二捕获基底上的互补捕获化合物来捕获所述至少一个测序梯; 以及从所述第二捕获基板去除所述至少一个测序梯。 第一和/或第二捕获基板可以包括磁性颗粒。 还公开了用于核酸样品制备的其它方法，工作流程，试剂盒和计算机程序培养基。