公开(公告)号：US20180362962A1

公开(公告)日：2018-12-20

申请号：US16011597

申请日：2018-06-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim G. BREVNOV ,  Hemant S. PAWAR ,  Manohar R. FURTADO ,  Jaiprakash G. SHEWALE

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

公开(公告)号：US20240425909A1

公开(公告)日：2024-12-26

申请号：US18701789

申请日：2022-10-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Say Li KONG ,  San Min LEOW ,  Andy YING ,  Manohar R. FURTADO ,  Grace Yu Hui WONG ,  Daryn KENNY ,  Jacelyn Hong Yi PHUA ,  Xiaohan XU

IPC: C12Q1/6851 ,  C12Q1/70

Abstract: Disclosed are compositions, kits, and methods for amplifying and quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the quantification of a target nucleic acid from a sample using an internal quantification standard disposed in the same reaction volume as the target nucleic acid, thereby eliminating the need for additional amplification reactions and processing to generate a standard curve. Compositions, kits, and methods also enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.

公开(公告)号：US20210246438A1

公开(公告)日：2021-08-12

申请号：US17181103

申请日：2021-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim G. BREVNOV ,  Hemant S. PAWAR ,  Manohar R. FURTADO ,  Jaiprakash G. SHEWALE

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.