公开(公告)号：US11905557B2

公开(公告)日：2024-02-20

申请号：US17193232

申请日：2021-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Swati Goyal ,  Achim Karger ,  Peter Ma ,  S. Jeffrey Rosner ,  Ian Walton ,  Jonathan Wang ,  Michael Wenz

IPC: C12Q1/6869 ,  C12Q1/6806 ,  B01L3/00

CPC classification number: C12Q1/6869 ,  B01L3/502753 ,  C12Q1/6806 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/16

Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

公开(公告)号：US20170051344A1

公开(公告)日：2017-02-23

申请号：US15113786

申请日：2015-01-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Swati Goyal ,  Achim Karger ,  Peter Ma ,  S. Jeffrey Rosner ,  Ian Walton ,  Jonathan Wang ,  Michael Wenz

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/6869 ,  B01L3/502753 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/16 ,  C12Q1/6806

Abstract: According to various embodiments described herein, a microfiuidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfiuidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfiuidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

Abstract translation: 根据本文描述的各种实施方案，提供了一种用于Sanger-测序反应的基于微型芯片的纯化装置和系统。 该装置和系统允许引入包含特定于测序污染物或测序方法的纯化技术的柱的测序系统，其中盒的简化纯化溶液允许样品纯化过程的自动化，净化试剂的消耗降低和一致性 通过减少采样误差和伪像进行采样。 因此，这些各种实施方案解决了对于用于CE器件的基于微流化芯片的Sanger-测序反应纯化系统的需求。 所描述的微流体芯片可以通过重组片上试剂，反应孔和工作流程步骤用作PCR芯片。