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公开(公告)号：US11104944B2
公开(公告)日：2021-08-31
申请号：US16247267
申请日：2019-01-14
Applicant: LIFE TECHNOLOGIES CORPORATION
IPC: C12Q1/68 , C12Q1/686 , C12Q1/6874 , C12Q1/6853 , C12Q1/6869
Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
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公开(公告)号：US20190211373A1
公开(公告)日：2019-07-11
申请号：US16247267
申请日：2019-01-14
Applicant: LIFE TECHNOLOGIES CORPORATION
IPC: C12Q1/686 , C12Q1/6874 , C12Q1/6869
CPC classification number: C12Q1/686 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
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公开(公告)号：US12006536B2
公开(公告)日：2024-06-11
申请号：US17399449
申请日：2021-08-11
Applicant: LIFE TECHNOLOGIES CORPORATION
IPC: C12Q1/68 , C12Q1/6853 , C12Q1/686 , C12Q1/6869 , C12Q1/6874
CPC classification number: C12Q1/686 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
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4.发明授权公开(公告)号：US11905557B2
公开(公告)日：2024-02-20
申请号：US17193232
申请日：2021-03-05
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Swati Goyal , Achim Karger , Peter Ma , S. Jeffrey Rosner , Ian Walton , Jonathan Wang , Michael Wenz
IPC: C12Q1/6869 , C12Q1/6806 , B01L3/00
CPC classification number: C12Q1/6869 , B01L3/502753 , C12Q1/6806 , B01L2200/0631 , B01L2200/10 , B01L2200/141 , B01L2300/0887 , B01L2300/12 , B01L2300/16
Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.
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Patent Agency Ranking
5.发明申请公开(公告)号：US20250019757A1
公开(公告)日：2025-01-16
申请号：US18405788
申请日：2024-01-05
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Swati Goyal , Jonathan Wang , Hans Wenz , Peter Ma , Achim Karger , S. Jeffrey Rosner , Ian Walton
IPC: C12Q1/6869 , B01L3/00 , C12Q1/6806
Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.
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公开(公告)号：US09708650B2
公开(公告)日：2017-07-18
申请号：US14198308
申请日：2014-03-05
Applicant: LIFE TECHNOLOGIES CORPORATION
CPC classification number: C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
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7.发明申请Purification Chemistries and Formats for Sanger DNA Sequencing Reactions on a Micro-Fluidics Device 审中-公开净化化学品和格式的微流体装置上的Sanger DNA测序反应公开(公告)号：US20170051344A1
公开(公告)日：2017-02-23
申请号：US15113786
申请日：2015-01-23
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Swati Goyal , Achim Karger , Peter Ma , S. Jeffrey Rosner , Ian Walton , Jonathan Wang , Michael Wenz
CPC classification number: C12Q1/6869 , B01L3/502753 , B01L2200/0631 , B01L2200/10 , B01L2200/141 , B01L2300/0887 , B01L2300/12 , B01L2300/16 , C12Q1/6806
Abstract: According to various embodiments described herein, a microfiuidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfiuidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfiuidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.
Abstract translation: 根据本文描述的各种实施方案，提供了一种用于Sanger-测序反应的基于微型芯片的纯化装置和系统。 该装置和系统允许引入包含特定于测序污染物或测序方法的纯化技术的柱的测序系统，其中盒的简化纯化溶液允许样品纯化过程的自动化，净化试剂的消耗降低和一致性 通过减少采样误差和伪像进行采样。 因此，这些各种实施方案解决了对于用于CE器件的基于微流化芯片的Sanger-测序反应纯化系统的需求。 所描述的微流体芯片可以通过重组片上试剂，反应孔和工作流程步骤用作PCR芯片。
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公开(公告)号：US09410192B2
公开(公告)日：2016-08-09
申请号：US14053571
申请日：2013-10-14
Applicant: LIFE TECHNOLOGIES CORPORATION
CPC classification number: C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
Abstract translation: 提供了一种组合物，其包含带负电荷的基团，寡核苷酸序列和至少一个或一个核酸酶抗性连锁基团以形成化学增强的引物。 化学增强引物可用于测序和片段分析。 还提供了合成引物的方法以及用于测序的DNA的制备方法和测序DNA的方法以及含有化学增强的引物的试剂盒。 DNA测序的方法可以包括使扩增反应产物与组合物在过量扩增引物被核酸酶降解的条件下接触，化学增强引物基本上不降解。
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公开(公告)号：US20140377747A1
公开(公告)日：2014-12-25
申请号：US14198308
申请日：2014-03-05
Applicant: LIFE TECHNOLOGIES CORPORATION
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
Abstract translation: 提供化学增强的引物，其包含带负电荷的部分（NCM），具有a）非核酸酶抗性核苷酸间连接的寡核苷酸序列或b）至少一个核酸酶抗性核苷酸间连接。 化学增强引物可用于测序和片段分析。 还提供了用于合成化学增强引物的方法以及制备用于测序的DNA的方法，测序DNA的方法和含有化学增强的引物的试剂盒。 测序DNA的方法可以包括使扩增反应产物与其中过量扩增引物被核酸酶降解并且化学增强引物基本上未降解的组合物接触。
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