公开(公告)号：US20190292515A1

公开(公告)日：2019-09-26

申请号：US16067326

申请日：2016-12-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mwita PHELPS ,  Paul GULDE ,  Richard FIKE ,  Mary REYNOLDS ,  Richard HASSETT

IPC: C12N1/22 ,  C12N5/00 ,  B01J2/00 ,  B01J2/16

Abstract: The present invention is directed to dry cell culture media or feeds comprising layered particles. The less stable or sensitive components are separated spatially from reactive components in media, feeds, supplements, or concentrates due to layering. The invention relates to processes for preparing such layered compositions and methods of making cell culture compositions that are stable, thermally, photo-chemically and/or to gamma irradiation. The invention also relates to kits and culture systems using media layered particles.

公开(公告)号：US20220372435A1

公开(公告)日：2022-11-24

申请号：US17761879

申请日：2020-09-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mwita PHELPS

IPC: C12N5/00

Abstract: Described herein are efficiently dissolving tablets of dry cell culture media, feeds, supplements, media subgroups, buffer concentrates, or media components useful in culturing cells or microorganisms, methods of manufacturing, and methods of use. In particular, formulations of tableted cell culture media feeds, supplements, media subgroups, or buffer concentrates are described.

公开(公告)号：US20210009942A1

公开(公告)日：2021-01-14

申请号：US17037454

申请日：2020-09-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mwita PHELPS ,  Paul GULDE ,  Richard FIKE ,  Mary REYNOLDS ,  Richard HASSETT ,  Andrew CAMPBELL

IPC: C12N5/00 ,  C12N1/22 ,  B01J8/18

Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. The media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.

公开(公告)号：US20210171901A1

公开(公告)日：2021-06-10

申请号：US16764692

申请日：2017-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Neva ALLES ,  Deirdre EYCHNER ,  Gabrielle LORENZO ,  Mary REYNOLDS ,  Paul GULDE ,  Ryan BONIFACE ,  Mwita PHELPS ,  Matthew KAHN ,  Richard FIKE

IPC: C12N5/00

Abstract: Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

公开(公告)号：US20180142203A1

公开(公告)日：2018-05-24

申请号：US15566899

申请日：2016-12-16

Applicant: Life Technologies Corporation

Inventor： Mwita PHELPS ,  Paul GULDE ,  Richard FIKE ,  Mary REYNOLDS ,  Richard HASSETT ,  Andrew CAMPBELL

IPC: C12N5/00 ,  C12N1/22 ,  B01J8/18

Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.