公开(公告)号：US20210009942A1

公开(公告)日：2021-01-14

申请号：US17037454

申请日：2020-09-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mwita PHELPS ,  Paul GULDE ,  Richard FIKE ,  Mary REYNOLDS ,  Richard HASSETT ,  Andrew CAMPBELL

IPC: C12N5/00 ,  C12N1/22 ,  B01J8/18

Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. The media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.

公开(公告)号：US20160376548A1

公开(公告)日：2016-12-29

申请号：US15198237

申请日：2016-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard FIKE ,  Shawn BARRETT ,  Zhou JIANG ,  David JUDD

IPC: C12N5/00 ,  B01J13/22 ,  B01J13/06

CPC classification number: C12N5/005 ,  B01J13/06 ,  B01J13/22 ,  B29C67/24 ,  B29L2009/00 ,  C12N5/0018 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2511/00 ,  C12N2533/40 ,  C12N2533/74

Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

公开(公告)号：US20150079659A1

公开(公告)日：2015-03-19

申请号：US14285047

申请日：2014-05-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen GORFIEN ,  Richard FIKE ,  GLENN GODWIN ,  JOYCE WOLANSKE ,  David EPSTEIN ,  Dale GRUBER ,  Don MCCLURE ,  Paul PRICE

IPC: C12N5/071 ,  C12N9/24

CPC classification number: C12N5/0682 ,  C07K14/61 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0603 ,  C12N7/00 ,  C12N9/2402 ,  C12N9/2471 ,  C12N15/85 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2710/10051 ,  C12Y302/01023

Abstract: The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

公开(公告)号：US20210269767A1

公开(公告)日：2021-09-02

申请号：US17307234

申请日：2021-05-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard FIKE ,  Bruce BRANCHAUD ,  Shawn BARRETT

IPC: C12N5/00

Abstract: Methods for increasing the stability of, or protecting, labile components, such as ethanolamine, growth factors, vitamins, etc., in compositions such as a cell culture medium, are described. Stability of the labile compound is increased either, by derivatization or by sequestering the labile compound. Sequestering can be done either by encapsulation within a microcapsule or by the use of sequestering agents. Encapsulation can provide controlled release of the susceptible compound being protected. These methods may improve or extend the storage conditions, the shipping and handling of media compositions comprising the labile compounds at room temperature rather than at lower temperatures, thereby decreasing shipping costs.

公开(公告)号：US20190292515A1

公开(公告)日：2019-09-26

申请号：US16067326

申请日：2016-12-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mwita PHELPS ,  Paul GULDE ,  Richard FIKE ,  Mary REYNOLDS ,  Richard HASSETT

IPC: C12N1/22 ,  C12N5/00 ,  B01J2/00 ,  B01J2/16

Abstract: The present invention is directed to dry cell culture media or feeds comprising layered particles. The less stable or sensitive components are separated spatially from reactive components in media, feeds, supplements, or concentrates due to layering. The invention relates to processes for preparing such layered compositions and methods of making cell culture compositions that are stable, thermally, photo-chemically and/or to gamma irradiation. The invention also relates to kits and culture systems using media layered particles.

公开(公告)号：US20210171901A1

公开(公告)日：2021-06-10

申请号：US16764692

申请日：2017-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Neva ALLES ,  Deirdre EYCHNER ,  Gabrielle LORENZO ,  Mary REYNOLDS ,  Paul GULDE ,  Ryan BONIFACE ,  Mwita PHELPS ,  Matthew KAHN ,  Richard FIKE

IPC: C12N5/00

Abstract: Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

公开(公告)号：US20200332250A1

公开(公告)日：2020-10-22

申请号：US16918838

申请日：2020-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard FIKE ,  Shawn BARRETT ,  Zhou JIANG ,  David JUDD

IPC: C12N5/00 ,  B29C67/24 ,  B01J13/06 ,  B01J13/22

Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

公开(公告)号：US20180346872A1

公开(公告)日：2018-12-06

申请号：US16054601

申请日：2018-08-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard FIKE ,  Shawn BARRETT ,  Zhou JIANG ,  David JUDD

IPC: C12N5/00 ,  B01J13/22 ,  B29C67/24 ,  B01J13/06 ,  B29L9/00

CPC classification number: C12N5/005 ,  B01J13/06 ,  B01J13/22 ,  B29C67/24 ,  B29L2009/00 ,  C12N5/0018 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2511/00 ,  C12N2533/40 ,  C12N2533/74

Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

公开(公告)号：US20180142203A1

公开(公告)日：2018-05-24

申请号：US15566899

申请日：2016-12-16

Applicant: Life Technologies Corporation

Inventor： Mwita PHELPS ,  Paul GULDE ,  Richard FIKE ,  Mary REYNOLDS ,  Richard HASSETT ,  Andrew CAMPBELL

IPC: C12N5/00 ,  C12N1/22 ,  B01J8/18

Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.