公开(公告)号：US20140066612A1

公开(公告)日：2014-03-06

申请号：US13964036

申请日：2013-08-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gulilat GEBEYEHU ,  Richard Pires

IPC: C12Q1/68

CPC classification number: C07H21/00 ,  C07B2200/11 ,  C12Q1/68

Abstract: The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.

Abstract translation: 本发明涉及一种通过使阻断的可检测标记的寡核苷酸与有效量的亲核性氨基化合物在导致寡核苷酸的实际解锁的条件下接触来封闭基本上封闭的可检测标记的寡核苷酸的方法，从而得到基本上解锁的寡核苷酸。

公开(公告)号：US20160024138A1

公开(公告)日：2016-01-28

申请号：US14748930

申请日：2015-06-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gulilat GEBEYEHU ,  Richard Pires

IPC: C07H21/00

CPC classification number: C07H21/00 ,  C07B2200/11 ,  C12Q1/68

Abstract: The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.

Abstract translation: 本发明涉及一种通过使阻断的可检测标记的寡核苷酸与有效量的亲核性氨基化合物在导致寡核苷酸的实际解锁的条件下接触来封闭基本上封闭的可检测标记的寡核苷酸的方法，从而得到基本上解锁的寡核苷酸。

公开(公告)号：US09085797B2

公开(公告)日：2015-07-21

申请号：US13964036

申请日：2013-08-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gulilat Gebeyehu ,  Richard Pires

IPC: C07H21/00 ,  C12Q1/68

CPC classification number: C07H21/00 ,  C07B2200/11 ,  C12Q1/68

Abstract: The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.