公开(公告)号：US20240425909A1

公开(公告)日：2024-12-26

申请号：US18701789

申请日：2022-10-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Say Li KONG ,  San Min LEOW ,  Andy YING ,  Manohar R. FURTADO ,  Grace Yu Hui WONG ,  Daryn KENNY ,  Jacelyn Hong Yi PHUA ,  Xiaohan XU

IPC: C12Q1/6851 ,  C12Q1/70

Abstract: Disclosed are compositions, kits, and methods for amplifying and quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the quantification of a target nucleic acid from a sample using an internal quantification standard disposed in the same reaction volume as the target nucleic acid, thereby eliminating the need for additional amplification reactions and processing to generate a standard curve. Compositions, kits, and methods also enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.