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    Regulatable promoter
    2.
    发明授权
    Regulatable promoter 有权
    可调节启动子

    公开(公告)号：US09512432B2

    公开(公告)日：2016-12-06

    申请号：US14350352

    申请日：2012-10-05

    申请人： LONZA LTD

    发明人： Diethard Mattanovich ,  Brigitte Gasser ,  Michael Maurer ,  Roland Prielhofer ,  Joachim Klein ,  Jana Wenger

    IPC分类号： C12P21/00 ,  C12N15/63 ,  C07K14/39 ,  C12N15/81 ,  C12N15/67 ,  C07K16/00

    CPC分类号： C12N15/815 ,  C07K14/39 ,  C07K16/00 ,  C07K2317/55 ,  C12N9/0008 ,  C12N15/635 ,  C12N15/67 ,  C12N15/81 ,  C12P21/00 ,  C12Y102/01012

    摘要： A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.

    摘要翻译： 一种通过培养包含表达构建体的重组真核生物细胞系（包括可调节启动子和编码所述启动子的转录控制下的POI的核酸分子）的重组真核生物细胞系的方法，包括以下步骤：a）培养细胞 与抑制启动子的基础碳源连接，b）用限制量的补充碳源培养细胞系，抑制启动子以与天然存在相比以至少15％的转录速率诱导POI的产生 pGAP启动子，和c）生产和回收POI; 并进一步分离可调节的启动子和相应的表达系统。

    Regulatable promoter
    4.
    发明授权
    Regulatable promoter 有权

    公开(公告)号：US10301634B2

    公开(公告)日：2019-05-28

    申请号：US15335132

    申请日：2016-10-26

    申请人： LONZA LTD.

    发明人： Diethard Mattanovich ,  Brigitte Gasser ,  Michael Maurer ,  Roland Prielhofer ,  Joachim Klein ,  Jana Wenger

    IPC分类号： C07K14/39 ,  C12N15/81 ,  C12N15/67 ,  C07K16/00 ,  C12N15/63 ,  C12P21/00 ,  C12N9/02

    摘要： A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.

    REGULATABLE PROMOTER
    5.
    发明申请
    REGULATABLE PROMOTER 有权
    可调节的促销者

    公开(公告)号：US20140242636A1

    公开(公告)日：2014-08-28

    申请号：US14350352

    申请日：2012-10-05

    申请人： LONZA LTD.

    发明人： Diethard Mattanovich ,  Brigitte Gasser ,  Michael Maurer ,  Roland Prielhofer ,  Joachim Klein ,  Jana Wenger

    IPC分类号： C12N15/63 ,  C07K16/00 ,  C12P21/00

    CPC分类号： C12N15/815 ,  C07K14/39 ,  C07K16/00 ,  C07K2317/55 ,  C12N9/0008 ,  C12N15/635 ,  C12N15/67 ,  C12N15/81 ,  C12P21/00 ,  C12Y102/01012

    摘要： A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.

    摘要翻译： 一种通过培养包含表达构建体的重组真核生物细胞系（包括可调节启动子和编码所述启动子的转录控制下的POI的核酸分子）的重组真核生物细胞系的方法，包括以下步骤：a）培养细胞 与抑制启动子的基础碳源连接，b）用限制量的补充碳源培养细胞系，抑制启动子以与天然存在相比以至少15％的转录速率诱导POI的产生 pGAP启动子，和c）生产和回收POI; 并进一步分离可调节的启动子和相应的表达系统。