公开(公告)号：US06183955B2

公开(公告)日：2001-02-06

申请号：US09014241

申请日：1998-01-27

申请人： Leif Andersson ,  Maria Johansson Moller ,  Richard Wales ,  Kenneth William Siggens ,  Graham Stuart Plastow

发明人： Leif Andersson ,  Maria Johansson Moller ,  Richard Wales ,  Kenneth William Siggens ,  Graham Stuart Plastow

IPC分类号： C12Q168

CPC分类号： C12Q1/6876 ,  C12Q1/6827 ,  C12Q2600/124 ,  C12Q2600/156 ,  C12Q2600/16 ,  Y10S435/81

摘要： Coat color is important to the pig breeding industry for a number of reasons. It is therefore desirable to develop populations of pigs which will breed true for coat color. However, establishing such populations would be time-consuming and costly using traditional test mating programs. It is therefore desirable to determine the coat color genotype of individual pigs. The inventors have shown that the KIT gene in pigs is involved with coat color determination. Specifically, the inventors have discovered that the difference between the I, IP, and i alleles of the coat color determining gene is duplication of at least part of the KIT gene in the I and IP alleles. Further, the inventors have discovered that the difference between the I and IP alleles is that, although both I and IP have a duplication in the KIT gene, only I and not IP exhibits a deletion in one of the duplicated regions. These discoveries have allowed the inventors to develop methods for distinguishing between the alleles I, IP, and i, thereby determining the genotype of individual pigs with respect to coat color. In accordance with the present invention, there are provided methods for determining the genotype of individual pigs with respect to coat color and kits for use in carrying out such methods.

摘要翻译： 由于种种原因，大衣颜色对养猪业很重要。 因此，需要开发出将种植真皮用于外套颜色的猪群。 然而，使用传统的测试交配程序，建立这样的人口将是耗时和昂贵的。 因此，需要确定个体猪的外套颜色基因型。 发明人已经表明，猪中的KIT基因涉及外壳颜色测定。 具体地，本发明人已经发现，外层颜色确定基因的I，IP和i等位基因之间的差异是I和IP等位基因中至少部分KIT基因的重复。 此外，发明人已经发现，I和IP等位基因之间的区别在于，虽然I和IP在KIT基因中都有重复，但只有I而不是IP在其中一个复制区域中表现出缺失。 这些发现允许发明人开发区分等位基因I，IP和i的方法，由此确定单个猪相对于衣服颜色的基因型。 根据本发明，提供了确定各种猪相对于外套颜色的基因型的方法和用于实施这些方法的试剂盒。