公开(公告)号：US20120102591A1

公开(公告)日：2012-04-26

申请号：US13264620

申请日：2010-04-14

申请人： Lizhong Xiong ,  Xin Hou ,  Zhuyun Qi

发明人： Lizhong Xiong ,  Xin Hou ,  Zhuyun Qi

IPC分类号： C12N15/82

CPC分类号： C07K14/415 ,  C12N15/8273

摘要： The present invention pertains to the field of rice genetic engineering. Specifically, the present invention relates to a rice OsNHAD gene that enhances tolerance to salt stress, which was obtained through gene isolation, cloning and function verification, and also to use of the gene in genetic improvement of salt tolerance of rice. Said gene is selected from one of the following nucleotide sequences: 1) the nucleotide sequence from positions 60 to 1649 of SEQ NO: 1 in the Sequence Listing; or 2) a nucleotide sequence that encodes the same protein as that encoded by 1). Transgenic rice plants obtained by introducing into rice the nucleotide sequence comprising OsNHAD gene operably ligated with exogenous promoter had enhanced salt tolerance.

摘要翻译： 本发明涉及水稻基因工程领域。 具体地，本发明涉及通过基因分离，克隆和功能验证获得的增强耐盐胁迫的水稻OsNHAD基因，并且还涉及该基因在水稻耐盐性遗传改良中的应用。 所述基因选自以下核苷酸序列之一：1）序列表中SEQ ID NO：1的位置60至1649的核苷酸序列; 或2）编码与1）编码的蛋白相同的蛋白质的核苷酸序列。 通过引入水稻获得的转基因水稻，包含可操作地与外源启动子连接的OsNHAD基因的核苷酸序列具有增强的耐盐性。

公开(公告)号：US20120102598A1

公开(公告)日：2012-04-26

申请号：US13319534

申请日：2010-05-12

申请人： Lizhong Xiong ,  Kabin Xie ,  Xin Hou

发明人： Lizhong Xiong ,  Kabin Xie ,  Xin Hou

IPC分类号： A01H1/06 ,  C12N5/10 ,  C12N15/82

CPC分类号： C12N15/8287 ,  A01H5/02 ,  A01H6/1424 ,  C12N15/8262

摘要： A rice microRNA, miR164 gene, that controls plant root system development and fertility, is obtained through gene isolation, cloning and function verification. Uses of a nucleic acid fragment comprising miR164, which fragment may confer a transformed plant with the ability to increase root number and to alter fertility, wherein the said nucleic acid fragment is selected from one of the following nucleotide sequences: 1) a DNA sequence as shown in SEQ ID NO: 1; 2) a RNA sequence as shown in SEQ ID NO:2; or 3) the conserved sequence of miR164 having the same function as 1) or 2). The nucleotide sequence containing the precursor of miR164 is ligated with an exogenous promoter and introduced into rice to obtain transgenic rice plants which has large root systems but became infertile. The fertility can be restored by external application of phytohormones.

摘要翻译： 通过基因分离，克隆和功能验证获得了控制植物根系发育和育性的水稻microRNA，miR164基因。 使用包含miR164的核酸片段，该片段可赋予具有增加根数和改变生育能力的能力的转化植物，其中所述核酸片段选自以下核苷酸序列之一：1）DNA序列作为 如SEQ ID NO：1所示; 2）如SEQ ID NO：2所示的RNA序列; 或3）具有与1）或2）相同功能的miR164的保守序列。 将含有miR164前体的核苷酸序列与外源启动子连接，并引入水稻中以获得具有大根系但变得不育的转基因水稻。 生殖力可以通过植物激素的外部应用来恢复。

公开(公告)号：US08802929B2

公开(公告)日：2014-08-12

申请号：US13319534

申请日：2010-05-12

申请人： Lizhong Xiong ,  Kabin Xie ,  Xin Hou

发明人： Lizhong Xiong ,  Kabin Xie ,  Xin Hou

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/113

CPC分类号： C12N15/8287 ,  A01H5/02 ,  A01H6/1424 ,  C12N15/8262

摘要： A rice microRNA, miR164 gene, that controls plant root system development and fertility, is obtained through gene isolation, cloning and function verification. Uses of a nucleic acid fragment comprising miR164, which fragment may confer a transformed plant with the ability to increase root number and to alter fertility, wherein the said nucleic acid fragment is selected from one of the following nucleotide sequences: 1) a DNA sequence as shown in SEQ ID NO: 1; 2) a RNA sequence as shown in SEQ ID NO:2; or 3) the conserved sequence of miR164 having the same function as 1) or 2). The nucleotide sequence containing the precursor of miR164 is ligated with an exogenous promoter and introduced into rice to obtain transgenic rice plants which has large root systems but became infertile. The fertility can be restored by external application of phytohormones.

摘要翻译： 通过基因分离，克隆和功能验证获得了控制植物根系发育和育性的水稻microRNA，miR164基因。 使用包含miR164的核酸片段，该片段可赋予具有增加根数和改变生育能力的能力的转化植物，其中所述核酸片段选自下列核苷酸序列之一：1）将DNA序列作为 如SEQ ID NO：1所示; 2）如SEQ ID NO：2所示的RNA序列; 或3）具有与1）或2）相同功能的miR164的保守序列。 将含有miR164前体的核苷酸序列与外源启动子连接，并引入水稻中以获得具有大根系但变得不育的转基因水稻。 生殖力可以通过植物激素的外部应用来恢复。

公开(公告)号：US20100186108A1

公开(公告)日：2010-07-22

申请号：US12531001

申请日：2008-03-11

申请人： Honghong Hu ,  Lizhong Xiong

发明人： Honghong Hu ,  Lizhong Xiong

IPC分类号： A01H5/00 ,  C12N15/82

CPC分类号： C12N15/8273 ,  C07K14/415 ,  C07K14/4702

摘要： The present invention relates to clone isolation, function confirmation and use of the SNAC2 gene from rice associated with the plant tolerance to cold and salt stress. Said gene comprises (a) a DNA sequence as shown in position 112-1023 of SEQ ID NO:1, or (b) a DNA sequence that encodes the same protein as that encoded by (a). The present invention also relates to use of said gene in increasing the tolerance of plants to drought and salt stress.

摘要翻译： 本发明涉及与植物耐寒和盐胁迫相关的水稻的SNAC2基因的克隆分离，功能确认和使用。 所述基因包含（a）如SEQ ID NO：1的位置112-1023所示的DNA序列，或（b）编码与（a）编码的相同蛋白质的DNA序列。 本发明还涉及所述基因在增加植物对干旱和盐胁迫的耐受性方面的用途。

公开(公告)号：US20150218581A1

公开(公告)日：2015-08-06

申请号：US14111633

申请日：2012-04-16

申请人： Lizhong Xiong ,  Yonghua Tan

发明人： Lizhong Xiong ,  Yonghua Tan

IPC分类号： C12N15/82 ,  A01G9/00

CPC分类号： C12N15/8273 ,  A01G9/00 ,  C07K14/415

摘要： Rice OXHS4 gene controlling root growth and conferring enhanced drought resistance, and use thereof in genetic improvement of rice drought resistance is provided. OXHS4 gene is cloned using candidate gene screening method. Drought stress experiments at seedling stage and adult plant stage shows that overexpression of OXHS4 gene can improve drought resistance of transgenic rice, while the oxhs4 mutant exhibits sensitivity to drought, showing the function of said gene and the use thereof.

摘要翻译： 提供了控制根生长并提高抗旱性的稻米OXHS4基因及其在耐旱性遗传改良中的应用。 使用候选基因筛选方法克隆OXHS4基因。 幼苗期和成虫期的干旱胁迫实验表明，OXHS4基因的过表达可以改善转基因水稻的抗旱性，而oxhs4突变体对干旱表现出敏感性，表现出该基因的功能及其用途。

公开(公告)号：US20140150133A1

公开(公告)日：2014-05-29

申请号：US14112073

申请日：2012-04-20

申请人： Lizhong Xiong ,  Ning Tang

发明人： Lizhong Xiong ,  Ning Tang

IPC分类号： C12N15/82

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： A genetically modified OsbZIP46CA1 gene is provided. Use of the modified gene in controlling drought resistance of a plant such as rice, and plants and cells comprising the modified gene are also provided.

摘要翻译： 提供遗传修饰的OsbZIP46CA1基因。 还提供了修饰基因在控制诸如水稻等植物的抗旱性中的应用，以及包含修饰基因的植物和细胞。

公开(公告)号：US20140208456A1

公开(公告)日：2014-07-24

申请号：US14009373

申请日：2012-04-01

申请人： Lizhong Xiong ,  Jun You

发明人： Lizhong Xiong ,  Jun You

IPC分类号： C12N15/82

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： Rice OsSRO1C gene conferring enhanced drought resistance of a plant is provided, wherein the said gene encodes an amino acid sequences as set forth in SEQ ID NO: 2. DNA constructs, transgenic plants or cells comprising the OsSRO1C gene and the uses of the OsSRO1C gene in improving though resistance of a plant are also provided. The OsSRO1C gene controlling rice drought resistance is cloned by screening a rice T-DNA insertion mutant database and determination of expression level and identification of drought stress phenotype, which shows that the mutant is co-segregated with the drought susceptible phenotype.

摘要翻译： 提供赋予植物抗旱性的水稻OsSRO1C基因，其中所述基因编码如SEQ ID NO：2所示的氨基酸序列.DNA构建体，转基因植物或包含OsSRO1C基因的细胞和OsSRO1C基因的用途 在提高植物抗药性方面也有提高。 通过筛选水稻T-DNA插入突变体数据库，测定表达水平和鉴定干旱胁迫表型，克隆了耐旱稻田OsSRO1C基因，表明突变体与干旱易感基因表型共分离。

公开(公告)号：US07834244B2

公开(公告)日：2010-11-16

申请号：US11722298

申请日：2005-12-20

申请人： Honghong Hu ,  Lizhong Xiong

发明人： Honghong Hu ,  Lizhong Xiong

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C07K14/415 ,  C12N15/8273

摘要： The present invention relates to an isolated polynucleotide capable of giving a plant tolerance to drought and/or salt stress, which comprises a polynucleotide sequence as shown in SEQ ID NO:1, and to a promoter capable of giving a plant tolerance to drought and/or salt stress. The present invention also relates to an expression vector comprising the said polynucleotide and/or the said promoter, and to a host cell transformed or transfected by the said expression vector. The present invention further relates to a use of the said polynucleotide or promoter sequence in improvement of plant tolerance to drought and/or salt stress.

摘要翻译： 本发明涉及能够给予植物耐干旱和/或盐胁迫的分离的多核苷酸，其包含如SEQ ID NO：1所示的多核苷酸序列，以及能够给植物耐干旱和/ 或盐胁迫。 本发明还涉及包含所述多核苷酸和/或所述启动子的表达载体，以及由所述表达载体转化或转染的宿主细胞。 本发明还涉及所述多核苷酸或启动子序列改善植物对干旱和/或盐胁迫耐受性的用途。

公开(公告)号：US07345219B2

公开(公告)日：2008-03-18

申请号：US10768886

申请日：2004-01-31

申请人： Yinong Yang ,  Lizhong Xiong

发明人： Yinong Yang ,  Lizhong Xiong

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/87

CPC分类号： C12N9/1205 ,  C07H21/04 ,  C12N15/8271

摘要： The present invention relates to the mitogen-activated protein kinase called MAPK5. The rice MAPK5 gene, its protein and kinase activity were induced by abscisic acid, pathogen infection, wounding, drought, salt and cold temperature. However, suppression of MAPK5 expression and kinase activity in dsRNAi transgenic plants resulted in constitutive expression of pathogenesis-related genes such as PR-1 and PR-10 but enhanced resistance to fungal and bacterial pathogens. In contrast, overexpressed transgenic lines exhibited elevated MAPK5 kinase activity and increased tolerance to drought, salt and cold stresses. This invention provides methods for increasing tolerance to abiotic and biotic stress in plant using MAPK5.

摘要翻译： 本发明涉及称为MAPK5的丝裂原活化蛋白激酶。 水稻MAPK5基因，其蛋白和激酶活性由脱落酸，病原体感染，伤口，干旱，盐和冷温度诱导。 然而，dsRNAi转基因植物中MAPK5表达和激酶活性的抑制导致发病相关基因如PR-1和PR-10的组成型表达，但对真菌和细菌病原体的抗性增强。 相比之下，过表达的转基因品系表现出升高的MAPK5激酶活性和增加的对干旱，盐和冷应激的耐受性。 本发明提供了使用MAPK5增加植物中非生物和生物胁迫耐受性的方法。