公开(公告)号：US5821058A

公开(公告)日：1998-10-13

申请号：US361176

申请日：1994-12-21

申请人： Lloyd M. Smith ,  Leroy E. Hood ,  Michael W. Hunkapiller ,  Tim J. Hunkapiller ,  Charles R. Connell

发明人： Lloyd M. Smith ,  Leroy E. Hood ,  Michael W. Hunkapiller ,  Tim J. Hunkapiller ,  Charles R. Connell

IPC分类号： C12Q1/68 ,  G01N27/447 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  G01N27/44721 ,  G01N27/44726

摘要： A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing chemistry and permit the detection and characterization of the fragments as they are resolved by electrophoresis through a gel. Preferably four different fragment sets are tagged with the fluorophores fluorescein, Texas Red, tetramethyl rhodamine, and 7-nitrobenzofurazan. A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.

摘要翻译： 用于DNA测序操作中产生的DNA片段的电泳分析的方法，其中使用发色团或荧光团标记由测序化学产生的DNA片段，并允许片段在通过凝胶电泳分离时的检测和表征。 优选地，用荧光团荧光素，德克萨斯红，四甲基罗丹明和7-硝基苯并呋咱标记四个不同的片段组。 用于在DNA测序操作中产生的DNA片段的电泳分析系统，包括：发色团或荧光标记的DNA片段; 用于接触电泳凝胶的区域; 用于将所述标记的DNA片段引入所述区域的方法; 以及用于在所述标记的DNA片段移动通过所述凝胶时监测所述标记的DNA片段的光度测量装置。

公开(公告)号：US5747249A

公开(公告)日：1998-05-05

申请号：US361176

申请日：1994-12-21

申请人： Lloyd M. Smith ,  Leroy E. Hood ,  Michael W. Hunkapiller ,  Tim J. Hunkapiller ,  Charles R. Connell

发明人： Lloyd M. Smith ,  Leroy E. Hood ,  Michael W. Hunkapiller ,  Tim J. Hunkapiller ,  Charles R. Connell

IPC分类号： C12Q1/68 ,  G01N27/447 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  G01N27/44721 ,  G01N27/44726 ,  C12Q2563/107 ,  C12Q2535/101

摘要： A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing chemistry and permit the detection and characterization of the fragments as they are resolved by electrophoresis through a gel. Preferably four different fragment sets are tagged with the fluorophores fluorescein, Texas Red, tetramethyl rhodamine, and 7-nitro-benzofurazan. A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.

公开(公告)号：US5171534A

公开(公告)日：1992-12-15

申请号：US558312

申请日：1990-10-15

申请人： Lloyd M. Smith ,  Leroy E. Hood ,  Michael W. Hunkapiller ,  Tim J. Hunkapiller

发明人： Lloyd M. Smith ,  Leroy E. Hood ,  Michael W. Hunkapiller ,  Tim J. Hunkapiller

IPC分类号： C12Q1/68 ,  G01N27/447

CPC分类号： G01N27/44726 ,  C12Q1/6869 ,  G01N27/44721 ,  Y10T436/143333

摘要： A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.

摘要翻译： 用于在DNA测序操作中产生的DNA片段的电泳分析系统，包括：发色团或荧光标记的DNA片段; 用于接触电泳凝胶的区域; 用于将所述标记的DNA片段引入所述区域的方法; 以及用于在所述标记的DNA片段移动通过所述凝胶时监测所述标记的DNA片段的光度装置

公开(公告)号：US06200748B1

公开(公告)日：2001-03-13

申请号：US08484340

申请日：1995-06-07

申请人： Lloyd M. Smith ,  Leroy E. Hood ,  Michael W. Hunkapiller ,  Tim J. Hunkapiller ,  Charles R. Connell

发明人： Lloyd M. Smith ,  Leroy E. Hood ,  Michael W. Hunkapiller ,  Tim J. Hunkapiller ,  Charles R. Connell

IPC分类号： C12Q168

CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  G01N27/44721 ,  G01N27/44726 ,  C12Q2563/107 ,  C12Q2535/101

摘要： This invention provides a duplex comprising an oligonucleotide primer and a template, wherein the primer is coupled chemically to a chromophore or fluorophore so as to allow chain extension by a polymerase. In one embodiment, the primer is extended by a polymerase to generate the complement of the template. In a further embodiment, the extended primer is separated from the template for use in a number of methods, including sequencing reactions. Methods of generating these compositions of matter are further provided.

摘要翻译： 本发明提供了一种双链体，其包含寡核苷酸引物和模板，其中引物与发色团或荧光团化学偶联，以允许通过聚合酶进行链延伸。 在一个实施方案中，引物通过聚合酶延伸以产生模板的补体。 在另一个实施方案中，将延伸的引物与模板分离以用于多种方法，包括测序反应。 进一步提供产生这些物质组合物的方法。